Ngfr Immunohistochemistry Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:333013 Huang T, et al., Direct Interaction of Sox10 With Cadherin-19 Mediates Early Sacral Neural Crest Cell Migration: Implications for Enteric Nervous System Development Defects. Gastroenterology. 2022 Jan;162(1):179-192.e11
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7434130
Gene symbol:	Ngfr
Gene name:	nerve growth factor receptor (TNFR superfamily, member 16)

Antibody:

anti-NGFR (MAB365)

Results

1Ai ©

Specimen 1A +/+: embryonic day 10.5 (more

)
Note: Double labeled: red - p75 (Ngfr); green - Sox10 (assay MGI:7434129).

Structure	Cell Type	Level	Pattern	Image	Note
TS17: migrating neural crest	migratory neural crest cell	Present	Regionally restricted	1Ai	Expression in sacral neural crest cells along medial pathway and their pioneer (most ventrally located) cells.

1Aii ©

Specimen 1A Dom/+: embryonic day 10.5; Sox10^Dom/Sox10⁺ (more

)
Note: Double labeled: red - p75 (Ngfr); green - Sox10 (assay MGI:7434129).

Structure	Cell Type	Level	Pattern	Image	Note
TS17: migrating neural crest	migratory neural crest cell	Weak	Regionally restricted	1Aii	Significant reduction of expression in pioneer sacral neural crest cells in periaortic area.

1Aiii ©

Specimen 1A Dom/Dom: embryonic day 10.5; Sox10^Dom/Sox10^Dom (more

)
Note: Double labeled: red - p75 (Ngfr); green - Sox10 (assay MGI:7434129).

Structure	Cell Type	Level	Pattern	Image	Note
TS17: migrating neural crest	migratory neural crest cell	Weak	Regionally restricted	1Aiii	Significant reduction of expression in pioneer sacral neural crest cells in periaortic area.

1Di ©

Specimen 1D +/+: embryonic day 11.5 (more

)
Note: Double labeled: red - p75 (Ngfr); green - Sox10 (assay MGI:7434129).

Structure	Cell Type	Level	Pattern	Image	Note
TS19: pelvic ganglion	migratory neural crest cell	Present	Regionally restricted	1Di	Expression in sacral neural crest cells lateral to hindgut.

1Dii ©

Specimen 1D Dom/+: embryonic day 11.5; Sox10^Dom/Sox10⁺ (more

)
Note: Double labeled: red - p75 (Ngfr); green - Sox10 (assay MGI:7434129).

Structure	Cell Type	Level	Pattern	Image	Note
TS19: pelvic ganglion	migratory neural crest cell	Present	Regionally restricted	1Dii	Expression in sacral neural crest cells lateral to hindgut.

1Diii ©

Specimen 1D Dom/Dom: embryonic day 11.5; Sox10^Dom/Sox10^Dom (more

)
Note: Double labeled: red - p75 (Ngfr); green - Sox10 (assay MGI:7434129).

Structure	Level	Pattern	Image	Note
TS19: pelvic ganglion	Absent		1Diii	Neural crest cells not found in position of pelvic ganglion.

S2Ci ©

Specimen S2C +/+: embryonic day 12.5 (more

)
Note: Double labeled: red - p75 (Ngfr); green - Sox10 (assay MGI:7434129).

Structure	Cell Type	Level	Pattern	Image	Note
TS20: pelvic ganglion	migratory neural crest cell	Present	Regionally restricted	S2Ci	Expression in sacral neural crest cells

S2Cii ©

Specimen S2C Dom/+: embryonic day 12.5; Sox10^Dom/Sox10⁺ (more

)
Note: Double labeled: red - p75 (Ngfr); green - Sox10 (assay MGI:7434129).

Structure	Cell Type	Level	Pattern	Image	Note
TS20: pelvic ganglion	migratory neural crest cell	Weak	Regionally restricted	S2Cii	Signficantly fewer sacral neural crest cells than in wildtype.

S2Ciii ©

Specimen S2C Dom/Dom: embryonic day 12.5; Sox10^Dom/Sox10^Dom (more

)
Note: Double labeled: red - p75 (Ngfr); green - Sox10 (assay MGI:7434129).

Structure	Level	Pattern	Image	Note
TS20: pelvic ganglion	Absent		S2Ciii	No co-expression with Sox10.

S2Ei ©

Specimen S2E +/+: embryonic day 13.5 (more

)
Note: Double labeled: red - p75 (Ngfr); green - Sox10 (assay MGI:7434129).

Structure	Cell Type	Level	Pattern	Image	Note
TS21: pelvic ganglion	migratory neural crest cell	Present	Regionally restricted	S2Ei	Expression in sacral neural crest cells

S2Eii ©

Specimen S2E Dom/+: embryonic day 13.5; Sox10^Dom/Sox10⁺ (more

)
Note: Double labeled: red - p75 (Ngfr); green - Sox10 (assay MGI:7434129).

Structure	Cell Type	Level	Pattern	Image	Note
TS21: pelvic ganglion	migratory neural crest cell	Weak	Regionally restricted	S2Eii	Signficantly fewer sacral neural crest cells than in wildtype.

S2Eiii ©

Specimen S2E Dom/Dom: embryonic day 13.5; Sox10^Dom/Sox10^Dom (more

)
Note: Double labeled: red - p75 (Ngfr); green - Sox10 (assay MGI:7434129).

Structure	Level	Pattern	Image	Note
TS21: pelvic ganglion	Absent		S2Eiii	No co-expression with Sox10.

S3Ai ©

Specimen S3A Vagal level +/+: embryonic day 9.0 (more

)
Note: Double labeled: red - p75 (Ngfr); green - BrdU.

Structure	Cell Type	Level	Pattern	Image	Note
TS14: migrating neural crest	migratory neural crest cell	Present		S3Ai

S3Aii ©

Specimen S3A Vagal level Dom/Dom: embryonic day 9.0; Sox10^Dom/Sox10^Dom (more

)
Note: Double labeled: red - p75 (Ngfr); green - BrdU.

Structure	Cell Type	Level	Pattern	Image	Note
TS14: migrating neural crest	migratory neural crest cell	Present		S3Aii

S3Ci ©

Specimen S3C Vagal level +/+: embryonic day 9.0 (more

)
Note: Double labeled: red - p75 (Ngfr); green - TUNEL.

Structure	Cell Type	Level	Pattern	Image	Note
TS14: migrating neural crest	migratory neural crest cell	Present		S3Ci

S3Cii ©

Specimen S3C Vagal level Dom/Dom: embryonic day 9.0; Sox10^Dom/Sox10^Dom (more

)
Note: Double labeled: red - p75 (Ngfr); green - TUNEL.

Structure	Cell Type	Level	Pattern	Image	Note
TS14: migrating neural crest	migratory neural crest cell	Present		S3Cii

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