Gfap Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:283394 Hughes LJ, et al., Yap/Taz are required for establishing the cerebellar radial glia scaffold and proper foliation. Dev Biol. 2020 Jan 1;457(1):150-162
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7434589
Gene symbol:	Gfap
Gene name:	glial fibrillary acidic protein

Antibody:	anti-GFAP (Glial Fibrillary Acidic Protein) Ab-4 (RB-087)
Detection system:	Secondary antibody coupled to Cy3

Results

Specimen S1B: postnatal day 7 (more

)
Note: Double labeled: red - Gfap; green - Yap1 (assay MGI:7434448).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum ventricular layer	Bergmann glial cell	Present		S1B left lower , S1B left upper , S1B middle lower , S1B middle upper

S6C ©

Specimen S6C: postnatal day 8 (more

)
Note: Double labeled: red - Gfap; green - Calb1 (assay MGI:7434575).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Bergmann glial cell	Present		S6C

S6D ©

Specimen S6D: postnatal day 8; Tg(Gfap-cre)77.6Mvs/0, Wwtr1^tm1.1Eno/Wwtr1^tm1.1Eno, Yap1^tm1.1Eno/Yap1^tm1.1Eno (more

)
Note: Conditional mutant. Double labeled: red - Gfap; green - Calb1 (assay MGI:7434575).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Bergmann glial cell	Present		S6D	Similar as in the wild-type.

S6E ©

Specimen S6E: postnatal day 21; Tg(Gfap-cre)77.6Mvs/0, Wwtr1^tm1.1Eno/Wwtr1^tm1.1Eno, Yap1^tm1.1Eno/Yap1^tm1.1Eno (more

)
Note: Conditional mutant. Double labeled: red - Gfap; green - Yap1 (assay MGI:7434448).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum vermis lobule IX	Bergmann glial cell	Present		S6E

S6F ©

Specimen S6F: postnatal day 21; Tg(Gfap-cre)77.6Mvs/0, Wwtr1^tm1.1Eno/Wwtr1^tm1.1Eno, Yap1^tm1.1Eno/Yap1^tm1.1Eno (more

)
Note: Conditional mutant. Double labeled: red - Gfap; green - Yap1 (assay MGI:7434448).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum vermis lobule IX	Bergmann glial cell	Present		S6F	Similar as in the wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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