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Gene Expression Data
Assay Details
Assay
Reference: J:326689 Phan TP, et al., Upstream open reading frames control PLK4 translation and centriole duplication in primordial germ cells. Genes Dev. 2022 Jun 1;36(11-12):718-736
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7442648
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct4 [EPR17929] (ab181557)
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 4B E10.25 +/+: embryonic day 10.25 (more )
Structure Level Pattern Image Note
TS16: primordial germ cell of dorsal mesentery Present 4B E10.25 +/+
Specimen 4B E10.25 +/deltau: embryonic day 10.25; Plk4em1Ahol/Plk4+ (more )
Structure Level Pattern Image Note
TS16: primordial germ cell of dorsal mesentery Present 4B E10.25 +/deltau Similar as in the wild-type.
Specimen 4B E10.25 deltau/deltau: embryonic day 10.25; Plk4em1Ahol/Plk4em1Ahol (more )
Structure Level Pattern Image Note
TS16: primordial germ cell of dorsal mesentery Present 4B E10.25 deltau/deltau Similar as in the wild-type.
Specimen 4B E10.5 +/+: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: primordial germ cell of gonad Present 4B E10.5 +/+
Specimen 4B E10.5 +/deltau: embryonic day 10.5; Plk4em1Ahol/Plk4+ (more )
Structure Level Pattern Image Note
TS17: primordial germ cell of gonad Present 4B E10.5 +/deltau Similar as in the wild-type.
Specimen 4B E10.5 deltau/deltau: embryonic day 10.5; Plk4em1Ahol/Plk4em1Ahol (more )
Structure Level Pattern Image Note
TS17: primordial germ cell of gonad Present 4B E10.5 deltau/deltau Similar as in the wild-type.
Specimen 4C +/+: embryonic day 10.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+ (more )
Note: Triple labeled: red - Pou5f1; magenta - Tubg1 (assay MGI:7442562) and Tubg2 (assay MGI:7442686); green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Level Pattern Image Note
TS17: primordial germ cell of gonad Present 4C +/+
Specimen 4C deltau/deltau: embryonic day 10.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4em1Ahol (more )
Note: Triple labeled: red - Pou5f1; magenta - Tubg1 (assay MGI:7442562) and Tubg2 (assay MGI:7442686); green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Level Pattern Image Note
TS17: primordial germ cell of gonad Present 4C deltau/deltau Similar as in the wild-type. A small fraction had an increased number of centrin-GFP foci that were often observed in a "flower-like" pattern.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
MGI 6.24 		The Jackson Laboratory