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Gene Expression Data
Assay Details
Assay
Reference: J:326689 Phan TP, et al., Upstream open reading frames control PLK4 translation and centriole duplication in primordial germ cells. Genes Dev. 2022 Jun 1;36(11-12):718-736
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7442667
Gene symbol: Ddx4
Gene name: DEAD box helicase 4
Antibody: Anti-DDX4/MVH (ab13840)
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 4F XX +/+: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 4F XX +/+
Specimen 4F XX +/deltau: embryonic day 13.5; Plk4em1Ahol/Plk4+ (more )
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 4F XX +/deltau Reduced number of positive cells (summarized in Fig 4E).
Specimen 4F XX deltau/deltau: embryonic day 13.5; Plk4em1Ahol/Plk4em1Ahol (more )
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Weak 4F XX deltau/deltau An approximately sevenfold reduction compared with the wild-type (summarized in Fig. 4E).
Specimen 4F XY +/+: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 4F XY +/+
Specimen 4F XY +/deltau: embryonic day 13.5; Plk4em1Ahol/Plk4+ (more )
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 4F XY +/deltau Similar as in the wild-type.
Specimen 4F XY deltau/deltau: embryonic day 13.5; Plk4em1Ahol/Plk4em1Ahol (more )
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Weak 4F XY deltau/deltau An approximately fivefold reduction compared with the wild-type (summarized in Fig. 4E).
Specimen 4G +/+: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+ (more )
Note: Triple labeled: red - Ddx4; magenta - Tubg1 (assay MGI:7442562) and Tubg2 (assay MGI:7442686); green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 4G DDX4 +/+ , 4G merge +/+
Specimen 4G +/deltau: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4+ (more )
Note: Triple labeled: red - Ddx4; magenta - Tubg1 (assay MGI:7442562) and Tubg2 (assay MGI:7442686); green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 4G merge +/deltau , 4G DDX4 +/deltau An increased number of centrin-GFP foci was seen.
Specimen 4G deltau/deltau: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4em1Ahol (more )
Note: Triple labeled: red - Ddx4; magenta - Tubg1 (assay MGI:7442562) and Tubg2 (assay MGI:7442686); green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 4G DDX4 deltau/deltau , 4G merge deltau/deltau An increased number of centrin-GFP foci was seen.
Specimen 4J +/+: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 4J +/+
Specimen 4J +/deltau: embryonic day 13.5; Plk4em1Ahol/Plk4+ (more )
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 4J +/deltau Similar as in the wild-type.
Specimen 4J deltau/deltau: embryonic day 13.5; Plk4em1Ahol/Plk4em1Ahol (more )
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 4J deltau/deltau Presence of micronuclei was seen (increased by about 10-fold compared with the wild-type).
Specimen 5I XX: embryonic day 13.5; Plk4em1Ahol/Plk4em2.1Ahol (more )
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 5I XX Rescued germ cell number was seen.
Specimen 5I XY: embryonic day 13.5; Plk4em1Ahol/Plk4em2.1Ahol (more )
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 5I XY Rescued germ cell number was seen.
Specimen 5K +/+: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+ (more )
Note: Triple labeled: red - Ddx4; magenta - Tubg1 (assay MGI:7442562 and Tubg2 (assay MGI:7442686); green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 5K +/+ upper
Specimen 5K deltau/deltau: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4em1Ahol (more )
Note: Triple labeled: red - Ddx4; magenta - Tubg1 (assay MGI:7442562 and Tubg2 (assay MGI:7442686); green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 5K deltau/deltau upper Reduced number of positive cells.
Specimen 5K deltau/-: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4em2.1Ahol (more )
Note: Triple labeled: red - Ddx4; magenta - Tubg1 (assay MGI:7442562 and Tubg2 (assay MGI:7442686); green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present 5K deltau/- upper Rescued germ cell number was seen.
Specimen S4D +/+: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+ (more )
Note: Triple labeled: red - Ddx4; magenta - Tubg1 (assay MGI:7442562 and Tubg2 (assay MGI:7442686); green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present S4D +/+ lower , S4D +/+ upper
Specimen S4D deltau/deltau: embryonic day 13.5; Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev/Gt(ROSA)26Sor+, Plk4em1Ahol/Plk4em1Ahol (more )
Note: Triple labeled: red - Ddx4; magenta - Tubg1 (assay MGI:7442562 and Tubg2 (assay MGI:7442686); green - centrioles labeled with Gt(ROSA)26Sortm1.1(EGFP/CETN1)Sev.
Structure Level Pattern Image Note
TS21: primordial germ cell of gonad Present S4D deltau/deltau upper , S4D deltau/deltau lower Some positive cells with supernumerary centrioles exhibited multipolar mitotic spindles, suggesting that these cells could generate aneuploid daughter cells with micronuclei or abnormal nuclear shape.
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
MGI 6.24 		The Jackson Laboratory