Gene Expression Data
Assay Details
Assay
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Reference:
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J:333334
Mayo F, et al., Aquaporin-4 Expression Switches from White to Gray Matter Regions during Postnatal Development of the Central Nervous System. Int J Mol Sci. 2023 Feb 3;24(3)
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7442962
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Gene symbol:
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Aqp4
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Gene name:
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aquaporin 4
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Antibody:
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anti-Aqp4
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Detection system:
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Secondary antibody coupled to Cy3
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Results
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Specimen
4A P1:
postnatal day 1
(more )
Specimen
4A P1:
(close )
Genetic Background:
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C57BL/6
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Age:
|
postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS27: brain |
Trace |
Widespread |
4A P1
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Scarce expression in all brain regions.
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TS27: cerebral cortex |
Trace |
Regionally restricted |
4A P1
|
Cortical expression no longer restricted to ventral areas; expression begins to be marked laterally.
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Specimen
4A P3:
postnatal day 3
(more )
Specimen
4A P3:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 3
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Sex:
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Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: brain |
Trace |
Widespread |
4A P3
|
Scarce expression in all brain regions.
|
TS27: cerebral cortex |
Trace |
Regionally restricted |
4A P3
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Cortical expression no longer restricted to ventral areas; expression begins to be marked laterally.
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Specimen
4A P7:
postnatal day 7
(more )
Specimen
4A P7:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 7
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Sex:
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Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
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Specimen
4A P11:
postnatal day 11
(more )
Specimen
4A P11:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 11
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4A P20:
postnatal day 20
(more )
Specimen
4A P20:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 20
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4A P60:
postnatal day 60
(more )
Specimen
4A P60:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 60
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4A 18M:
postnatal month 18
(more )
Specimen
4A 18M:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal month 18
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5A P1:
postnatal day 1
(more )
Specimen
5A P1:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
postnatal day 1
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: cerebellum |
Trace |
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5A P1
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Cerebellum is very poorly developed and crest is barely recognizable; expression is very weak.
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Specimen
5A P3:
postnatal day 3
(more )
Specimen
5A P3:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
postnatal day 3
|
Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: cerebellum |
Trace |
|
5A P3
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Cerebellum is very poorly developed and crest is barely recognizable; expression is very weak.
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Specimen
5A P7:
postnatal day 7
(more )
Specimen
5A P7:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
postnatal day 7
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
Strong |
Regionally restricted |
5A P7
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High expression in white matter of cerebellum crest. Expression in deep part of cerebellar lobules, specifically in myelin-enriched internal branches.
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Specimen
5A P11:
postnatal day 11
(more )
Specimen
5A P11:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
postnatal day 11
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
Present |
Regionally restricted |
5A P11
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Same pattern of expression as at P7, but weaker in intensity.
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Specimen
5A P20:
postnatal day 20
(more )
Specimen
5A P20:
(close )
Genetic Background:
|
C57BL/6
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Age:
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postnatal day 20
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
5A P60:
postnatal day 60
(more )
Specimen
5A P60:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 60
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Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
Trace |
|
5A P60
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Very low levels of expression throughout adult life.
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Specimen
5A 18M:
postnatal month 18
(more )
Specimen
5A 18M:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
postnatal month 18
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Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebellum |
Trace |
|
5A 18M
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Very low levels of expression throughout adult life.
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Specimen
5B P1:
postnatal day 1
(more )
Specimen
5B P1:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: spinal cord lumbar region |
Present |
Regionally restricted |
5B P1
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Expression limited to edges of spinal cord. Few differences detected between gray matter and white matter.
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Specimen
5B P3:
postnatal day 3
(more )
Specimen
5B P3:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
postnatal day 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: spinal cord lumbar region |
Present |
Regionally restricted |
5B P3
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Expression in white matter begins to be more intense than in gray matter zone.
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Specimen
5B P7:
postnatal day 7
(more )
Specimen
5B P7:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spinal cord lumbar region |
Present |
Regionally restricted |
5B P7
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More intense peripheral expression coincident with external location of white matter.
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Specimen
5B P11:
postnatal day 11
(more )
Specimen
5B P11:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 11
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spinal cord lumbar region |
Present |
Regionally restricted |
5B P11
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Expression in white matter decreases significanlygly, reaching low levels observed in gray matterl.
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Specimen
5B P20:
postnatal day 20
(more )
Specimen
5B P20:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
postnatal day 20
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spinal cord lumbar region |
Present |
Regionally restricted |
5B P20
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Higher expression in gray matter in dorsal horns. Expression in white matter strongly reduced.
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Specimen
5B P60:
postnatal day 60
(more )
Specimen
5B P60:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
postnatal day 60
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spinal cord lumbar region |
Present |
Regionally restricted |
5B P60
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Higher expression in gray matter in dorsal horns. Expression in white matter strongly reduced.
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Specimen
5B 18M:
postnatal month 18
(more )
Specimen
5B 18M:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal month 18
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spinal cord lumbar region |
Present |
Regionally restricted |
5B 18M
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Higher expression in gray matter in dorsal horns. Expression in white matter strongly reduced.
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Specimen
6A:
postnatal day 7
(more )
Note: Double labeled: red - Aqp4; green - Gfap (assay MGI:7442965).
Specimen
6A:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 7
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Sex:
|
Not Specified
|
Type:
|
optical section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Aqp4; green - Gfap (assay MGI:7442965).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: corpus callosum |
glial cell |
Present |
Regionally restricted |
6A
,
6A inset
|
Larger population of cells co-expressing Gfap than at P60. Expression in cells with less mature glial cell phenotype (increased size and more nonspecific distribution of expression in cell membrane).
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Specimen
6B:
postnatal day 60
(more )
Note: Double labeled: red - Aqp4; green - Gfap (assay MGI:7442965).
Specimen
6B:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 60
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Aqp4; green - Gfap (assay MGI:7442965).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: corpus callosum |
astrocyte |
Present |
Regionally restricted |
6B
,
6B inset
|
Expression mainly polarized at interface between astrocyte endfeet processes and vessels.
|
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