Gene Expression Data
Assay Details
Assay
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Reference:
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J:333334
Mayo F, et al., Aquaporin-4 Expression Switches from White to Gray Matter Regions during Postnatal Development of the Central Nervous System. Int J Mol Sci. 2023 Feb 3;24(3)
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7442965
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Gene symbol:
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Gfap
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Gene name:
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glial fibrillary acidic protein
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Antibody:
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anti-Gfap
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
6A:
postnatal day 7
(more )
Note: Double labeled: green - Gfap; red - Aqp4 (assay MGI:7442962).
Specimen
6A:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Gfap; red - Aqp4 (assay MGI:7442962).
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: corpus callosum |
glial cell |
Present |
Regionally restricted |
6A
,
6A inset
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Larger population of cells co-expressing Gfap than at P60. Expression in cells with less mature glial cell phenotype (increased size and more nonspecific distribution of expression in cell membrane).
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Specimen
6B:
postnatal day 60
(more )
Note: Double labeled: green - Gfap; red - Aqp4 (assay MGI:7442962).
Specimen
6B:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 60
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Gfap; red - Aqp4 (assay MGI:7442962).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: corpus callosum |
astrocyte |
Present |
Regionally restricted |
6B
,
6B inset
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Expression mainly polarized at interface between astrocyte endfeet processes and vessels.
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