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Gene Expression Data
Assay Details
Assay
Reference: J:202344 Lesko MH, et al., Sox2 modulates the function of two distinct cell lineages in mouse skin. Dev Biol. 2013 Oct 1;382(1):15-26
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7447375
Gene symbol: Prdm1
Gene name: PR domain containing 1, with ZNF domain
Reporter: GFP
Antibody: anti-GFP (Invitrogen)
Detection system: Secondary antibody
Results
Specimen 1G: embryonic day 16.5; Prdm1tm1Nutt/Prdm1+ (more )
Note: Double labeled: green - Prdm1 reporter; red - Prdm1 protein (assay MGI:7447374).
Structure Level Pattern Image Note
TS24: epidermis suprabasal layer Present 1G Coexpressed with endogenous Prdm1/Blimp1.
TS24: hair follicle dermal papilla Present 1G Coexpressed with endogenous Prdm1/Blimp1 in the precursors of the dermal papillae.
Specimen 1H: embryonic day 18.5; Prdm1tm1Nutt/Prdm1+ (more )
Note: Double labeled: green - Prdm1 reporter; red - Prdm1 protein (assay MGI:7447374).
Structure Level Pattern Image Note
TS26: hair follicle dermal papilla Present 1H Coexpressed with endogenous Prdm1/Blimp1.
TS26: epidermis suprabasal layer Present 1H A progressive increase in expression was seen. Coexpressed with endogenous Prdm1/Blimp1.
Specimen 1I: postnatal day 2; Prdm1tm1Nutt/Prdm1+ (more )
Note: Double labeled: green - Prdm1 reporter; red - Prdm1 protein (assay MGI:7447374).
Structure Level Pattern Image Note
TS27: epidermis suprabasal layer Present 1I Coexpressed with endogenous Prdm1/Blimp1. A progressive increase in expression was seen.
TS27: hair follicle dermal papilla Present 1I Coexpressed with endogenous Prdm1/Blimp1 at a lower level compared with embryonic expression.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/10/2024
MGI 6.24 		The Jackson Laboratory