Antibody:	anti-Sox2
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS21: skin	Ambiguous		1A

Structure	Level	Pattern	Image	Note
TS24: hair follicle dermal papilla	Present		1B
TS24: epidermis	Absent		1B

Structure	Level	Pattern	Image	Note
TS26: hair follicle dermal papilla	Present		1C

Structure	Level	Pattern	Image	Note
TS27: hair follicle dermal papilla	Present		1D , 1D inset
TS27: epidermis	Absent		1D , 1D inset

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		5J	Coexpressed with Krt8 in most of the cells. The small population of Sox2+Krt8- cells could be Merkel cells in the initial phase of differentiation.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		6A	Coexpressed with Krt8 in most of the cells. The small population of Sox2+Krt8- cells could be Merkel cells in the initial phase of differentiation.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		6B	Coexpressed with Krt8 in most of the cells. The small population of Sox2+Krt8- cells could be Merkel cells in the initial phase of differentiation.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Absent		6C

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Absent		6D

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		6E	Coexpressed with Krt8 in most of the cells. The small population of Sox2+Krt8- cells could be Merkel cells in the initial phase of differentiation.
TS26: hair follicle dermal papilla		Present		6E

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Absent		6F
TS26: hair follicle dermal papilla		Present		6F

Structure	Cell Type	Level	Pattern	Image	Note
TS28: epidermis basal layer	Merkel cell	Present		6G

Structure	Cell Type	Level	Pattern	Image	Note
TS28: epidermis basal layer	Merkel cell	Present		6H

Structure	Cell Type	Level	Pattern	Image	Note
TS28: epidermis basal layer	Merkel cell	Absent		6I

Structure	Cell Type	Level	Pattern	Image	Note
TS28: epidermis basal layer	Merkel cell	Absent		6J

Structure	Cell Type	Level	Pattern	Image	Note
TS26: dermis		Weak	Regionally restricted	S1C	Some weakly positive cells were seen in the dermis. These could potentially be nerve cells.
TS26: hair follicle		Weak	Regionally restricted	S1C	Some weakly positive cells were seen around the hair follicles in the bulge area. These could potentially be nerve cells.
TS26: hair follicle dermal papilla		Present		S1C
TS26: epidermis basal layer	Merkel cell	Present		S1C	Coexpressed with Krt8 in most of the cells. The small population of Sox2+Krt14+Krt8- cells could be Merkel cells in the initial phase of differentiation.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: dermis		Weak	Regionally restricted	S1D	Some weakly positive cells were seen in the dermis. These could potentially be nerve cells.
TS26: hair follicle		Weak	Regionally restricted	S1D	Some weakly positive cells were seen around the hair follicles in the bulge area. These could potentially be nerve cells.
TS26: hair follicle dermal papilla		Present		S1D
TS26: epidermis basal layer	Merkel cell	Present		S1D	Coexpressed with Krt8 in most of the cells. The small population of Sox2+Krt14+Krt8- cells could be Merkel cells in the initial phase of differentiation.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		S1E	Coexpressed with Krt8 in most of the cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		S1F	Coexpressed with Krt8 in most of the cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: back skin	Merkel cell	Trace			Sox2 was successfully deleted, with only a small number of Sox2-positive cells detectable.
TS24: epidermis basal layer	Merkel cell	Trace			Sox2 was successfully deleted, with only a small number of Sox2-positive cells detectable.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: epidermis basal layer	Merkel cell	Trace			Sox2 was successfully deleted, with only a small number of Sox2-positive cells detectable.
TS24: mystacial pad	Merkel cell	Trace			Sox2 was successfully deleted, with only a small number of Sox2-positive cells detectable.