Krt8 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:202344 Lesko MH, et al., Sox2 modulates the function of two distinct cell lineages in mouse skin. Dev Biol. 2013 Oct 1;382(1):15-26
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7447388
Gene symbol:	Krt8
Gene name:	keratin 8

Antibody:	TROMA 1
Detection system:	Secondary antibody

Results

5B ©

Specimen 5B: postnatal day 2 (more

)
Note: Double labeled: green - Krt8; red - Nefh (assay MGI:7447390).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: epidermis basal layer	Merkel cell	Present		5B

5I ©

Specimen 5I: postnatal day 2 (more

)
Note: Double labeled: green - Krt8; red - Krt14 (assay MGI:7447381).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: epidermis basal layer	Merkel cell	Present		5I

5J ©

Specimen 5J: embryonic day 19.5 (more

)
Note: Double labeled: green - Krt8; red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		5J	Coexpressed with Sox2.

6A ©

Specimen 6A: embryonic day 18.5; Sox2^tm4.1Skn/Sox2⁺, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		6A

6B ©

Specimen 6B: embryonic day 18.5; Sox2^tm4.1Skn/Sox2⁺, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		6B

6C ©

Specimen 6C: embryonic day 18.5; Sox2^tm4.1Skn/Sox2^tm4.1Skn, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		6C	Some Merkel cells still differentiated.

6D ©

Specimen 6D: embryonic day 18.5; Sox2^tm4.1Skn/Sox2^tm4.1Skn, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		6D	Some Merkel cells still differentiated.

6E ©

Specimen 6E: embryonic day 18.5; Sox2^tm4.1Skn/Sox2⁺, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		6E

6F ©

Specimen 6F: embryonic day 18.5; Sox2^tm4.1Skn/Sox2^tm4.1Skn, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		6F	Some Merkel cells still differentiated.

6G ©

Specimen 6G: postnatal day 21; Sox2^tm4.1Skn/Sox2⁺, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: epidermis basal layer	Merkel cell	Present		6G

6H ©

Specimen 6H: postnatal day 21; Sox2^tm4.1Skn/Sox2⁺, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Triple labeled: green - Krt8; white - Krt14 (assay MGI:7447393); red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: epidermis basal layer	Merkel cell	Present		6H

6I ©

Specimen 6I: postnatal day 21; Sox2^tm4.1Skn/Sox2^tm4.1Skn, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: epidermis basal layer	Merkel cell	Present		6I	Some Merkel cells still differentiated.

6J ©

Specimen 6J: postnatal day 21; Sox2^tm4.1Skn/Sox2^tm4.1Skn, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Triple labeled: green - Krt8; white - Krt14 (assay MGI:7447393); red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: epidermis basal layer	Merkel cell	Present		6J	Some Merkel cells still differentiated.

7A ©

Specimen 7A: embryonic day 18.5; Sox2^tm4.1Skn/Sox2⁺, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		7A

7B ©

Specimen 7B: embryonic day 18.5; Sox2^tm4.1Skn/Sox2^tm4.1Skn, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		7B	Significant reduction in the number of positive Merkel cells per touch dome, compared with the control.

9A ©

Specimen 9A: embryonic day 18.5; Sox2^tm4.1Skn/Sox2⁺, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Uchl1 (assay MGI:7447394).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		9A

9E ©

Specimen 9E: embryonic day 18.5; Sox2^tm4.1Skn/Sox2⁺, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Uchl1 (assay MGI:7447394).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		9E

9F ©

Specimen 9F: embryonic day 18.5; Sox2^tm4.1Skn/Sox2⁺, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Uchl1 (assay MGI:7447394).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		9F

9G ©

Specimen 9G: embryonic day 18.5; Sox2^tm4.1Skn/Sox2^tm4.1Skn, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Uchl1 (assay MGI:7447394).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		9G	Reduction in the number of positive Merkel cells compared with the control, although with a similar innervated Merkel cells per touch dome.

S1C ©

Specimen S1C: embryonic day 18.5 (more

)
Note: Triple labeled: green - Krt8; blue - Krt14 (assay MGI:7447401); red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		S1C	Coexpressed with Sox2.

S1D ©

Specimen S1D: embryonic day 18.5 (more

)
Note: Triple labeled: green - Krt8; blue - Krt14 (assay MGI:7447401); red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		S1D	Coexpressed with Sox2.

S1E ©

Specimen S1E: embryonic day 19.5 (more

)
Note: Double labeled: green - Krt8; red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		S1E	Coexpressed with Sox2.

S1F ©

Specimen S1F: embryonic day 19.5 (more

)
Note: Double labeled: green - Krt8; red - Sox2 (assay MGI:7447377).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		S1F	Coexpressed with Sox2.

S2A ©

Specimen S2A: postnatal day 2 (more

)
Note: Double labeled: green - Krt8; red - Krt20 (assay MGI:7447402).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: epidermis basal layer	Merkel cell	Present		S2A	Coexpressed with Krt20.

S2B ©

S2B" ©

Specimen S2B/B": embryonic day 18.5 (more

)
Note: Double labeled: green - Krt8; red - Ncam1 (assay MGI:7447403).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		S2B" , S2B	Coexpressed with Ncam1 in some of the cells.

S3A ©

Specimen S3A: embryonic day 18.5; Sox2^tm4.1Skn/Sox2^tm4.1Skn (more

)
Note: Double labeled: green - Krt8; red - Mki67 (assay MGI:7447404).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		S3A

S3B ©

Specimen S3B: embryonic day 18.5; Sox2^tm4.1Skn/Sox2^tm4.1Skn, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Krt8; red - Mki67 (assay MGI:7447404).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		S3B	Reduced number of positive Merkel cells, compared with the control.

S3E ©

Specimen S3E: embryonic day 18.5; Sox2^tm4.1Skn/Sox2^tm4.1Skn (more

)
Note: Triple labeled: green - Krt8; white - Krt14 (assay MGI:7447393); red - Casp3 (assay MGI:7447405).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		S3E

S3F ©

Specimen S3F: embryonic day 18.5; Sox2^tm4.1Skn/Sox2^tm4.1Skn, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant. Triple labeled: green - Krt8; white - Krt14 (assay MGI:7447393); red - Casp3 (assay MGI:7447405).

Structure	Cell Type	Level	Pattern	Image	Note
TS26: epidermis basal layer	Merkel cell	Present		S3F	Reduced number of positive Merkel cells, compared with the control.

Specimen not shown: embryonic day 16.5; Sox2^tm4.1Skn/Sox2^tm4.1Skn, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: back skin	Merkel cell	Present
TS24: epidermis basal layer	Merkel cell	Present

Specimen not shown: embryonic day 16.5; Sox2^tm4.1Skn/Sox2^tm4.1Skn, Tg(KRT14-cre)1Amc/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: epidermis basal layer	Merkel cell	Present
TS24: mystacial pad	Merkel cell	Present

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