About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:330602 Zhou T, et al., Microglial debris is cleared by astrocytes via C4b-facilitated phagocytosis and degraded via RUBICON-dependent noncanonical autophagy in mice. Nat Commun. 2022 Oct 24;13(1):6233
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7451198
Gene symbol: Gfap
Gene name: glial fibrillary acidic protein
Antibody: anti-GFAP (clone G-A-5)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: The authors reported using two Gfap antibodies but did not specify which was used to obtain these data; it is possible MGI:5615208 was used.
Results
Specimen S1b - cortex: postnatal month 2; Cx3cr1tm1Litt/Cx3cr1+ (more )
Note: Double labeled: red/magenta - Gfap; green - Cx3cr1 reporter (assay MGI:7451215).
Structure Cell Type Level Pattern Image Note
TS28: cerebral cortex astrocyte Present S1b CD lower left , S1b CD cortex
Specimen S1b - hippocampus: postnatal month 2; Cx3cr1tm1Litt/Cx3cr1+ (more )
Note: Double labeled: red/magenta - Gfap; green - Cx3cr1 reporter (assay MGI:7451215).
Structure Cell Type Level Pattern Image Note
TS28: hippocampus astrocyte Present S1b CD lower center , S1b CD hippocampus
Specimen S1b - olfactory bulb: postnatal month 2; Cx3cr1tm1Litt/Cx3cr1+ (more )
Note: Double labeled: red/magenta - Gfap; green - Cx3cr1 reporter (assay MGI:7451215).
Structure Cell Type Level Pattern Image Note
TS28: main olfactory bulb astrocyte Present S1b CD lower right , S1b CD olfactory bulb
Specimen S9b - astrocyte: postnatal; P2ry12em1(cre/ERT2)Peng/P2ry12+ (more )
Note: Double labeled: red - Gfap; green - P2ry12 reporter (assay MGI:7450957).
Structure Cell Type Level Pattern Image Note
TS28: brain astrocyte Present S9b astrocyte
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
06/12/2024
MGI 6.13 		The Jackson Laboratory