Gene Expression Data
Assay Details
Assay
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Reference:
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J:49697
Belting HG, et al., Multiple phases of expression and regulation of mouse Hoxc8 during early embryogenesis. J Exp Zool. 1998 Sep-Oct 1;282(1-2):196-222
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7461127
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Gene symbol:
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Hoxc8
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Gene name:
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homeobox C8
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Antibody:
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C592/7E
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Detection system:
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Secondary antibody coupled to fluorescein
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Results
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Specimen
5A:
embryonic day 9.5
(more )
Specimen
5A:
(close )
Genetic Background:
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FVB
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5B:
embryonic day 9.5
(more )
Specimen
5B:
(close )
Genetic Background:
|
FVB
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
|
Specimen
5C:
embryonic day 10.5
(more )
Specimen
5C:
(close )
Genetic Background:
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FVB
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6A:
embryonic day 9.5
(more )
Specimen
6A:
(close )
Genetic Background:
|
FVB
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS15: embryo |
|
Present |
Regionally restricted |
6A
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Expression was found in tissues of ectodermal and mesodermal origin.
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TS15: migrating neural crest |
migratory neural crest cell |
Present |
|
6A
|
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Specimen
6B:
embryonic day 10.5
(more )
Specimen
6B:
(close )
Genetic Background:
|
FVB
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
6C:
embryonic day 11.5
(more )
Note: level of prevertebra 5
Specimen
6C:
(close )
Genetic Background:
|
FVB
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
level of prevertebra 5
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: neural tube |
Present |
Regionally restricted |
6C
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Expression was detected in a more anterior location up to the level of the 4th prevertebra. No expression was found in the ventral horns or ependymal layer at this axial level.
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TS19: neural tube mantle layer |
Present |
Regionally restricted |
6C
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Expression was detected in a more anterior location up to the level of the 4th prevertebra. In this region was limited to a population in the mediolateral region of the mantle layer.
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Specimen
6D:
embryonic day 11.5
(more )
Note: level of prevertebra 7
Specimen
6D:
(close )
Genetic Background:
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FVB
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Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
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Note:
|
level of prevertebra 7
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Structure |
Level |
Pattern |
Image |
Note |
TS19: neural tube |
Present |
Regionally restricted |
6D
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The posterior expression domain up to prevertebra 5 was consistent with a continuation of the pattern observed at E10.5. Expression was predominant in the ventral horns. Low levels of expression were found in the ependymal layer with an intensity gradient from ventral to dorsal.
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TS19: neural tube mantle layer |
Present |
Regionally restricted |
6D
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Expression expanded concomitantly in the ventral and mediolateral region of the mantle layer.
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Specimen
6E:
embryonic day 12.5
(more )
Note: level of prevertebra 3
Specimen
6E:
(close )
Genetic Background:
|
FVB
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
level of prevertebra 3
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Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Regionally restricted |
6E
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In proximity to the anterior boundary of Hoxc8 expression in the spinal cord, expression was in two stripes. A vertical band of cells expressing Hoxc8 extended along the ependymal layer in a dorsoventral orientation. A second, wedge-shaped group of cells was found in a medial to lateral orientation across the mantle layer.
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TS20: dorsal root ganglion |
Absent |
|
6E
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At this level of the spinal cord, the dorsal root ganglion was not stained.
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Specimen
6F:
embryonic day 12.5
(more )
Note: level of prevertebra 5
Specimen
6F:
(close )
Genetic Background:
|
FVB
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
level of prevertebra 5
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Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Regionally restricted |
6F
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Expression gradually returned to the ventral horns towards more posterior levels.
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Specimen
6G:
embryonic day 12.5
(more )
Note: level of prevertebra 8
Specimen
6G:
(close )
Genetic Background:
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FVB
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Age:
|
embryonic day 12.5
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
level of prevertebra 8
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Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Regionally restricted |
6G
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Expression was fully reconstituted at the level of the 7th prevertebra and persisted through the level of the 10th prevertebra.
|
TS20: dorsal root ganglion |
Present |
Regionally restricted |
6G
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At this level of the spinal cord, expression was also present in a subset of cells in dorsal root ganglia.
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Specimen
7A:
embryonic day 12.5
(more )
Note: Cross sections through embryos in the upper thoracic region (level of prevertebra 8). Double labeled: green - Hoxc8; red - Map2 (assay MGI:7461215).
Specimen
7A:
(close )
Genetic Background:
|
FVB
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Age:
|
embryonic day 12.5
|
Sex:
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Not Specified
|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
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Cross sections through embryos in the upper thoracic region (level of prevertebra 8). Double labeled: green - Hoxc8; red - Map2 (assay MGI:7461215).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS20: thoracic dorsal root ganglion |
|
Present |
Regionally restricted |
7A
,
7B
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Expression in the dorsal root ganglion coincided with the level of strong expression in the spinal cord.
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TS20: sympathetic ganglion |
neuron |
Present |
Regionally restricted |
7A
,
7B
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Expression within the sympathetic chain was restricted to the three to four anterior-most, upper thoracic ganglia. Co-labeling with Map2 indicated the staining was associated with differentiating neurons.
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TS20: spinal cord |
neuron |
Strong |
Regionally restricted |
7B
,
7A
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Expression was strong in the ventral horn of the spinal cord. Co-labeling with Map2 indicated the staining was associated with differentiating neurons.
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Specimen
7C:
embryonic day 12.5
(more )
Note: Cross sections through embryos in the upper thoracic region (level of prevertebra 8).
Specimen
7C:
(close )
Genetic Background:
|
FVB
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Cross sections through embryos in the upper thoracic region (level of prevertebra 8).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS20: sympathetic ganglion |
neuron |
Present |
Regionally restricted |
7C
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Expression was detected in differentiating neurons.
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Specimen
7E:
embryonic day 12.5
(more )
Note: Cross sections through embryos in the upper thoracic region (level of prevertebra 8). Double labeled: green - Hoxc8; red - Prph (assay MGI:7461216).
Specimen
7E:
(close )
Genetic Background:
|
FVB
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Cross sections through embryos in the upper thoracic region (level of prevertebra 8). Double labeled: green - Hoxc8; red - Prph (assay MGI:7461216).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS20: spinal cord mantle layer |
spinal cord motor neuron |
Present |
Regionally restricted |
7E
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Coexpression of Hoxc8 and Prph was found in a small subset of cells in the spinal cord at the outer border of the mantle layer. These cells are likely to be presynaptic motor neurons. Other sets of motor neurons expressed Hoxc8 alone.
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Specimen
7F:
embryonic day 12.5
(more )
Note: Cross sections through embryos in the upper thoracic region (level of prevertebra 8).
Specimen
7F:
(close )
Genetic Background:
|
FVB
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
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Cross sections through embryos in the upper thoracic region (level of prevertebra 8).
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Specimen
7G:
embryonic day 12.5
(more )
Note: Cross sections through embryos in the upper thoracic region (level of prevertebra 8). Double labeled: green - Hoxc8; red - Prph (assay MGI:7461216).
Specimen
7G:
(close )
Genetic Background:
|
FVB
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Cross sections through embryos in the upper thoracic region (level of prevertebra 8). Double labeled: green - Hoxc8; red - Prph (assay MGI:7461216).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS20: thoracic dorsal root ganglion |
neuron |
Present |
Regionally restricted |
7G
|
Colabeling of Hoxc8 and Prph was detected in differentiating neurons.
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