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Gene Expression Data
Assay Details
Assay
Reference: J:146199 Barrionuevo F, et al., Testis cord differentiation after the sex determination stage is independent of Sox9 but fails in the combined absence of Sox9 and Sox8. Dev Biol. 2009 Mar 15;327(2):301-12
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7464301
Gene symbol: H2ax
Gene name: H2A.X variant histone
Antibody: anti-gammaH2AX (Ser139) (05-636)
Detection system: Secondary antibody coupled to Cy2
Assay notes: Fixation with 3.7% formaldehyde.
Results
Specimen 1Da: postnatal month 7 (more )
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 1Da Expression in stage IX tubules with leptotene spermatocytes.
TS28: spermatocyte Present 1Da Expression in stage IX tubules with leptotene spermatocytes.
TS28: spermatid Weak 1Da Weaker expression in elongating spermatids.
Specimen 1Db: postnatal month 7; Sox9tm1Gsr/Sox9tm1Gsr, Plekha5Tg(AMH-cre)1Flor/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 1Db Expression in stage IX tubules with leptotene spermatocytes.
TS28: spermatocyte Present 1Db Expression in stage IX tubules with leptotene spermatocytes.
TS28: spermatid Weak 1Db Expression in a few elongating spermatids.
Specimen 1Dc: postnatal month 12; Sox9tm1Gsr/Sox9tm1Gsr, Plekha5Tg(AMH-cre)1Flor/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: seminiferous tubule Weak Regionally restricted 1Dc Expression in apoptotic bodies.
Specimen 1Dd: postnatal month 12; Sox9tm1Gsr/Sox9tm1Gsr, Plekha5Tg(AMH-cre)1Flor/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: seminiferous tubule Ambiguous 1Dd Authors describe only autofluorescence.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
MGI 6.13 		The Jackson Laboratory