About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:146199 Barrionuevo F, et al., Testis cord differentiation after the sex determination stage is independent of Sox9 but fails in the combined absence of Sox9 and Sox8. Dev Biol. 2009 Mar 15;327(2):301-12
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7464304
Gene symbol: Gcna
Gene name: germ cell nuclear acidic protein
Antibody: TRA98
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Fixation with 3.7% formaldehyde.
Results
Specimen 1Ea: postnatal month 5 (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Present Regionally restricted 1Ea Expression in ordered spermatogenic cells.
Specimen 1Eb: postnatal month 5; Sox9tm1Gsr/Sox9tm1Gsr, Plekha5Tg(AMH-cre)1Flor/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: germ cell of testis Present Regionally restricted 1Eb Some tubules completely void of spermatogenic cells.
Specimen 7Ca: postnatal day 6 (more )
Structure Level Pattern Image Note
TS28: spermatogonium Present 7Ca Expression in prepuberal spermatogonia located at periphery.
Specimen 7Cb: postnatal day 12 (more )
Structure Level Pattern Image Note
TS28: spermatocyte Present 7Cb Abundant expressing spermatocytes at P12.
Specimen 7Cc: postnatal day 18 (more )
Structure Level Pattern Image Note
TS28: spermatocyte Present 7Cc Abundant expressing spermatocytes at P18.
Specimen 7Cg: postnatal day 6; Sox8tm1Weg/Sox8tm1Weg, Sox9tm1Gsr/Sox9tm1Gsr, Plekha5Tg(AMH-cre)1Flor/Plekha5+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: spermatogonium Present 7Cg Expression in prepuberal spermatogonia located at periphery similar to control.
Specimen 7Ch: postnatal day 12; Sox8tm1Weg/Sox8tm1Weg, Sox9tm1Gsr/Sox9tm1Gsr, Plekha5Tg(AMH-cre)1Flor/Plekha5+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: spermatocyte Weak 7Ch Expression less abundant compared to control.
Specimen 7Ci: postnatal day 18; Sox8tm1Weg/Sox8tm1Weg, Sox9tm1Gsr/Sox9tm1Gsr, Plekha5Tg(AMH-cre)1Flor/Plekha5+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: seminiferous tubule Weak Regionally restricted 7Ci Authors report an aberrant pattern of expression in few remaining tubules.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
06/12/2024
MGI 6.13 		The Jackson Laboratory