Tjp1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:226036 Maddala R, et al., Rap1 GTPase is required for mouse lens epithelial maintenance and morphogenesis. Dev Biol. 2015 Oct 1;406(1):74-91
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7464648
Gene symbol:	Tjp1
Gene name:	tight junction protein 1

Antibody:	anti-ZO1
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

4B E13.5 WT ©

Specimen 4B E13.5 WT: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: lens epithelium	Present		4B E13.5 WT	Expressed discretely at the apical junctions of epithelium and primary fiber cells.

4B E13.5 cKO ©

Specimen 4B E13.5 cKO: embryonic day 13.5; Rap1a^tm1Morz/Rap1a^tm1Morz, Rap1b^tm1Morz/Rap1b^tm1Morz, Tg(Pax6-cre,GFP)1Pgr/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS21: lens epithelium	Trace		4B E13.5 cKO	Positive cell adhesive interactions were dramatically reduced to almost completely absent.

4B E15.5 WT ©

Specimen 4B E15.5 WT: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: lens epithelium	Strong		4B E15.5 WT	Expressed discretely at the apical junctions of epithelium and primary fiber cells. Distributed intensely at the lens fulcrum region.

4B E15.5 cKO ©

Specimen 4B E15.5 cKO: embryonic day 15.5; Rap1a^tm1Morz/Rap1a^tm1Morz, Rap1b^tm1Morz/Rap1b^tm1Morz, Tg(Pax6-cre,GFP)1Pgr/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS23: lens epithelium	Trace		4B E15.5 cKO	Positive cell adhesive interactions were dramatically reduced to almost completely absent.

4B E17.5 WT ©

Specimen 4B E17.5 WT: embryonic day 17.5 (more

)

Structure	Level	Pattern	Image	Note
TS25: lens epithelium	Strong		4B E17.5 WT	Expressed discretely at the apical junctions of epithelium and primary fiber cells. Distributed intensely at the lens fulcrum region.

4B E17.5 cKO ©

Specimen 4B E17.5 cKO: embryonic day 17.5; Rap1a^tm1Morz/Rap1a^tm1Morz, Rap1b^tm1Morz/Rap1b^tm1Morz, Tg(Pax6-cre,GFP)1Pgr/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS25: lens epithelium	Trace		4B E17.5 cKO	Positive cell adhesive interactions were dramatically reduced to almost completely absent.

Specimen not shown: embryonic day 15.5; Tg(Pax6-cre,GFP)1Pgr/0 (more

)

Structure	Level	Pattern	Image	Note
TS23: lens epithelium	Present			Similar as in the wild-type.

Specimen not shown: embryonic day 17.5; Tg(Pax6-cre,GFP)1Pgr/0 (more

)

Structure	Level	Pattern	Image	Note
TS25: lens epithelium	Present			Similar as in the wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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