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Gene Expression Data
Assay Details
Assay
Reference: J:271281 Bowles J, et al., Retinoic Acid Antagonizes Testis Development in Mice. Cell Rep. 2018 Jul 31;24(5):1330-1341
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7466615
Gene symbol: Ddx4
Gene name: DEAD box helicase 4
Antibody: Anti-DDX4/MVH (ab27591)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 3A Wt AMH: embryonic day 13.5 (more )
Note: Double labeled: red - Ddx4; green - Amh (assay MGI:7466639).
Structure Level Pattern Image Note
TS21: germ cell of testis Present 3A XY Wt left , 3A XY Wt left inset

Specimen 3A KO AMH: embryonic day 13.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd (more )
Note: Double labeled: red - Ddx4; green - Amh (assay MGI:7466639).
Structure Level Pattern Image Note
TS21: germ cell of gonad Present 3A XY KO left , 3A XY KO left inset

Specimen 3A Wt FOXL2: embryonic day 13.5 (more )
Note: Double labeled: red - Ddx4; green - Foxl2 (assay MGI:7466640).
Structure Level Pattern Image Note
TS21: germ cell of testis Present 3A XY Wt right , 3A XY Wt right inset

Specimen 3A KO FOXL2: embryonic day 13.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd (more )
Note: Double labeled: red - Ddx4; green - Foxl2 (assay MGI:7466640).
Structure Level Pattern Image Note
TS21: germ cell of gonad Present 3A XY KO right , 3A XY KO right inset

Specimen 4A Wt SCC: embryonic day 13.5 (more )
Note: Double labeled: red - Ddx4; green - Cyp11a1 (assay MGI:7466641).
Structure Level Pattern Image Note
TS21: germ cell of testis Present 4A XY Wt left , 4A XY Wt left inset

Specimen 4A KO SCC: embryonic day 13.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd (more )
Note: Double labeled: red - Ddx4; green - Cyp11a1 (assay MGI:7466641).
Structure Level Pattern Image Note
TS21: germ cell of gonad Present 4A XY KO left , 4A XY KO left inset

Specimen 4A Wt HSD3B: embryonic day 14.5 (more )
Note: Double labeled: red - Ddx4; green - Hsd3b (assay MGI:7466642).
Structure Level Pattern Image Note
TS22: germ cell of testis Present 4A XY Wt right inset , 4A XY Wt right

Specimen 4A KO HSD3B: embryonic day 14.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd (more )
Note: Double labeled: red - Ddx4; green - Hsd3b (assay MGI:7466642).
Structure Level Pattern Image Note
TS22: germ cell of gonad Present 4A XY KO right inset , 4A XY KO right

Specimen 5B Wt: embryonic day 14.5 (more )
Note: Double labeled: red - Ddx4; green - Sox9 (assay MGI:7466659).
Structure Level Pattern Image Note
TS22: germ cell of testis Present 5B XY Wt inset , 5B XY Wt

Specimen 5B KO: embryonic day 14.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd (more )
Note: Double labeled: red - Ddx4; green - Sox9 (assay MGI:7466659).
Structure Level Pattern Image Note
TS22: germ cell of gonad Present 5B XY KO , 5B XY KO inset


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
06/12/2024
MGI 6.13
The Jackson Laboratory