Gene Expression Data
Assay Details
Assay
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Reference:
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J:171712
Bird AD, et al., cAMP response element binding protein is required for differentiation of respiratory epithelium during murine development. PLoS One. 2011;6(3):e17843
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7466645
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Gene symbol:
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Creb1
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Gene name:
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cAMP responsive element binding protein 1
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Antibody:
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anti-phospho-CREB
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
3C:
embryonic day 16.5
(more )
Specimen
3C:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS24: lung |
Present |
Regionally restricted |
3C
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Sporadic expression in almost all cells of lung
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TS24: lung mesenchyme |
Present |
|
3C
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Sparce expression in mesenchyme.
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Specimen
3C Proximal:
embryonic day 16.5
(more )
Specimen
3C Proximal:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3C Distal:
embryonic day 16.5
(more )
Specimen
3C Distal:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3D:
embryonic day 18.5
(more )
Specimen
3D:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: lung |
Weak |
Regionally restricted |
3D
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Expression in distal lung epithelium much reduced.
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TS26: lung mesenchyme |
Present |
Regionally restricted |
3D
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Expression primarily in interstitial mesenchyme surrounding large airways.
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Specimen
3D Proximal:
embryonic day 18.5
(more )
Specimen
3D Proximal:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: lung mesenchyme |
Present |
Regionally restricted |
3D Proximal
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Increased mesenchymal expression surrounding proximal epithelium.
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Specimen
3D Distal:
embryonic day 18.5
(more )
Specimen
3D Distal:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
|