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Gene Expression Data
Assay Details
Assay
Reference: J:335085 Baral K, et al., APJ+ cells in the SHF contribute to the cells of aorta and pulmonary trunk through APJ signaling. Dev Biol. 2023 Apr 8;498:77-86
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7467519
Gene symbol: Tnnt2
Gene name: troponin T2, cardiac
Antibody: CT3
Detection system: Secondary antibody
Results
Specimen 4A Control: embryonic day 9.5; Aplnrtm1.2Tq/Aplnrtm1.2Tq (more )
Note: Double labeled: red - cTnT (Tnnt2); green - phospho-histone-3 (PHH3).
Structure Cell Type Level Pattern Image Note
TS15: secondary heart field cardiac muscle cell Present 4A Control
Specimen 4A Islet1Cre+: embryonic day 9.5; Aplnrtm1.2Tq/Aplnrtm1.2Tq, Isl1tm1(cre)Sev/Isl1+ (more )
Note: Conditional mutant. Double labeled: red - cTnT (Tnnt2); green - phospho-histone-3 (PHH3).
Structure Cell Type Level Pattern Image Note
TS15: secondary heart field cardiac muscle cell Present 4A Islet1Cre+ No difference in number of expressing cells within second heart field region compared with control.
Specimen 4C Control: embryonic day 9.5; Aplnrtm1.2Tq/Aplnrtm1.2Tq (more )
Note: Double labeled: red - cTnT (Tnnt2); green - phospho-histone-3 (PHH3).
Structure Cell Type Level Pattern Image Note
TS15: secondary heart field cardiac muscle cell Present 4C Control
Specimen 4C Mef2cCre+: embryonic day 9.5; Aplnrtm1.2Tq/Aplnrtm1.2Tq, Tg(Mef2c-cre)2Blk/0 (more )
Note: Conditional mutant. Double labeled: red - cTnT (Tnnt2); green - phospho-histone-3 (PHH3).
Structure Cell Type Level Pattern Image Note
TS15: secondary heart field cardiac muscle cell Present 4C Mef3cCre+ No difference in number of expressing cells within second heart field region compared with control.
Specimen S1A Control: embryonic day 9.5; Aplnrtm1.2Tq/Aplnrtm1.2Tq (more )
Note: Double labeled: red - cTnT (Tnnt2); green - TUNEL.
Structure Cell Type Level Pattern Image Note
TS15: secondary heart field cardiac muscle cell Present S1A Control
Specimen S1A Mef2cCre+: embryonic day 9.5; Aplnrtm1.2Tq/Aplnrtm1.2Tq, Tg(Mef2c-cre)2Blk/0 (more )
Note: Conditional mutant. Double labeled: red - cTnT (Tnnt2); green - TUNEL.
Structure Cell Type Level Pattern Image Note
TS15: secondary heart field cardiac muscle cell Present S1A Mef2cCre+
Specimen S2A Control: embryonic day 13.5; Aplnrtm1.2Tq/Aplnrtm1.2Tq (more )
Note: Double labeled: blue - cTnT (Tnnt2); red - VE-Cadherin (Cdh5 assay MGI:7467520).
Structure Cell Type Level Pattern Image Note
TS21: heart cardiac muscle cell Present S2A Control (upper) , S2A Control (middle)
Specimen S2A Mef2cCre+: embryonic day 13.5; Aplnrtm1.2Tq/Aplnrtm1.2Tq, Tg(Mef2c-cre)2Blk/0 (more )
Note: Conditional mutant. Double labeled: blue - cTnT (Tnnt2); red - VE-Cadherin (Cdh5 assay MGI:7467520).
Structure Cell Type Level Pattern Image Note
TS21: heart cardiac muscle cell Present S2A Mef2cCre+ (middle) , S2A Mef2cCre+ (upper)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/22/2024
MGI 6.24 		The Jackson Laboratory