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Gene Expression Data
Assay Details
Assay
Reference: J:333828 Blommers M, et al., Retinal neuroblast migration and ganglion cell layer organization require the cytoskeletal-interacting protein Mllt11. Dev Dyn. 2023 Feb;252(2):305-319
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7469655
Gene symbol: Sox2
Gene name: SRY (sex determining region Y)-box 2
Antibody: anti-Sox2
Detection system: Secondary antibody coupled to Alexa Fluor 568
Results
Specimen 4A/G: embryonic day 18.5 (more )
Note: Double labeled: yellow - Sox2; magenta - Pax2 (assay MGI:7469656).
Structure Cell Type Level Pattern Image Note
TS26: retina ganglion cell layer amacrine cell Present Regionally restricted 4A , 4G Expressed in the basal boundary.
TS26: retina layer Present Regionally restricted 4A , 4G Expressed throughout the neuroblastic layer as well as in a subset of cells positioned in both the inner nuclear layer and basal boundary of the ganglion cell layer.
TS26: retina inner nuclear layer amacrine cell Present Scattered 4G , 4A
TS26: retina ganglion cell layer retinal astrocyte Present Scattered 4A , 4G Coexpressed with Pax2.
Specimen 4B/H: embryonic day 18.5; Mllt11tm1c(KOMP)Mbp/Mllt11tm1c(KOMP)Mbp, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Cux2tm1.1(cre)Mull/Cux2+ (more )
Note: Conditional mutant. Double labeled: yellow - Sox2; magenta - Pax2 (assay MGI:7469656).
Structure Cell Type Level Pattern Image Note
TS26: retina ganglion cell layer amacrine cell Present Single cells 4B , 4H Reduced positive cells (Sox2+Pax2-) in the basal region compared with the control.
TS26: retina inner nuclear layer amacrine cell Present Scattered 4B , 4H The mutant had more ectopically placed cells caught in the inner nuclear layer/inner plexiform layer region.
TS26: retina ganglion cell layer retinal astrocyte Present Single cells 4B , 4H Relative distribution of Sox2+Pax2+ cells was similar as in the control.
TS26: retina layer Present Regionally restricted 4B , 4H Expressed throughout the neuroblastic layer. More positive cells in the inner nuclear layer/inner plexiform layer and significant fewer in the basal-most edge of the ganglion cell layer.
Specimen 6J/J': embryonic day 18.5 (more )
Note: Double labeled: yellow - Sox2; magenta - EdU.
Structure Cell Type Level Pattern Image Note
TS26: retina ganglion cell layer amacrine cell Present 6J' , 6J Expressed in the displaced amacrine cells.
TS26: retina inner nuclear layer amacrine cell Present 6J' , 6J Expressed in the displaced amacrine cells.
Specimen 6K/K': embryonic day 18.5; Mllt11tm1c(KOMP)Mbp/Mllt11tm1c(KOMP)Mbp, Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/Gt(ROSA)26Sor+, Cux2tm1.1(cre)Mull/Cux2+ (more )
Note: Conditional mutant. Double labeled: yellow - Sox2; magenta - EdU.
Structure Cell Type Level Pattern Image Note
TS26: retina ganglion cell layer amacrine cell Present 6K , 6K' Expressed in the displaced amacrine cells. Fewer Sox2+ EdU+ cells in the basal-most regions compared with controls.
TS26: retina inner nuclear layer amacrine cell Present 6K' , 6K Expressed in the displaced amacrine cells. More Sox2+ EdU+ cells relative to controls.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
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