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Gene Expression Data
Assay Details
Assay
Reference: J:213958 Lang RA, et al., p120-catenin-dependent junctional recruitment of Shroom3 is required for apical constriction during lens pit morphogenesis. Development. 2014 Aug;141(16):3177-87
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7486716
Gene symbol: Myh10
Gene name: myosin, heavy polypeptide 10, non-muscle
Antibody: Anti-Myosin IIB (PRB-445P)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1A left: embryonic day 10.0 (more )
Note: Double labeled: red - Myh10; green - Ctnnb1 (assay MGI:7486717).
Structure Level Pattern Image Note
TS16: lens placode Strong 1A left Intense staining in the apical junctions, colocalized with zonula adheren protein Ctnnb1.
Specimen 1C peripheral: embryonic day 10.0 (more )
Structure Level Pattern Image Note
TS16: lens placode Strong 1C peripheral left Intense staining in the apical junctions. Placodal cells contained one or several actomyosin filaments and, in the latter case, they were orientated in approximately the same direction.
Specimen 1C central: embryonic day 10.0 (more )
Structure Level Pattern Image Note
TS16: lens placode Strong 1C center left Intense staining in the apical junctions.
Specimen 1F: embryonic day 10.0 (more )
Note: Double labeled: red - Myh10; green - Ctnnb1 (assay MGI:7486717).
Structure Level Pattern Image Note
TS16: lens placode Present 1F Also localized to apically positioned filament-like structures spanning the apical cortex, which were Ctnnb1 negative.
Specimen 1G: embryonic day 10.0 (more )
Note: Double labeled: red - Myh10; green - Ctnnb1 (assay MGI:7486717).
Structure Level Pattern Image Note
TS16: lens placode Present 1G Also localized to apically positioned filament-like structures spanning the apical cortex, which were Ctnnb1 negative.
Specimen 1J: embryonic day 10.0 (more )
Note: Double labeled: red - Myh10; green - Ctnnb1 (assay MGI:7486717).
Structure Level Pattern Image Note
TS16: head surface ectoderm Present 1J Filaments appeared to be under tension or appeared to traverse the junctions were not usually found in the non-apically constricting cells.
Specimen 1L: embryonic day 10.0 (more )
Note: Double labeled: red - Myh10; green - Ctnnb1 (assay MGI:7486717).
Structure Level Pattern Image Note
TS16: olfactory placode Present 1L Filaments appeared to be under tension or appeared to traverse the junctions were also observed in cells within the periphery.
Specimen 1M: embryonic day 10.0 (more )
Structure Level Pattern Image Note
TS16: olfactory placode Present 1M Filaments appeared to be under tension or appeared to traverse the junctions were also observed in cells within the periphery.
Specimen 1O: embryonic day 10.0 (more )
Note: Double labeled: red - Myh10; green - Ctnnb1 (assay MGI:7486717).
Structure Level Pattern Image Note
TS16: olfactory placode Present 1O Filaments appeared to be under tension or appeared to traverse the junctions were also observed in cells within the periphery, coincident with junctional displacement.
Specimen 5A left: embryonic day 10.0; Ctnnd1tm1Abre/Ctnnd1+ (more )
Note: Double labeled: red - Myh10; green - Ctnnb1 (assay MGI:7486717).
Structure Level Pattern Image Note
TS16: lens placode Present 5A left myo IIb , 5A left merge Locally displaced bicellular junctions were seen. The displacement was significantly greater than the width of a straight filament-nonassociated junction (see Fig. 5B).
Specimen 5A right: embryonic day 10.0; Ctnnd1tm1Abre/Ctnnd1+, Shroom3Gt(ROSA53)Sor/Shroom3+ (more )
Note: Conditional mutant. Double labeled: red - Myh10; green - Ctnnb1 (assay MGI:7486717).
Structure Level Pattern Image Note
TS16: lens placode Present 5A right merge , 5A right myo IIb Filament-associated bicellular junctions did not curve and on average were not significantly displaced (see Fig. 5B).
Specimen 5K: embryonic day 10.5; Ctnnd1tm1Abre/Ctnnd1tm1Abre (more )
Structure Level Pattern Image Note
TS17: lens pit Present 5K
Specimen 5L: embryonic day 10.5; Ctnnd1tm1Abre/Ctnnd1tm1Abre, Tfap2atm1(cre)Moon/Tfap2a+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: lens pit Present 5L Quantifiably reduced in the apical junctions of hinge point cells, but apical localization in the central portion of the lens pit is unchanged.
Specimen 5M: embryonic day 10.5; Ctnnd1tm1Abre/Ctnnd1+, Shroom3Gt(ROSA53)Sor/Shroom3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: lens pit Present 5M Significant reduction in hinge point cells. Lower (but not significantly) expression in the central cells.
Specimen S3B: embryonic day 10.0 (more )
Note: Double labeled: red - Myh10; green - Ctnnd1 (assay MGI:7486721).
Structure Level Pattern Image Note
TS16: lens placode Present S3B middle , S3B merge
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/10/2024
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