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Gene Expression Data
Assay Details
Assay
Reference: J:213958 Lang RA, et al., p120-catenin-dependent junctional recruitment of Shroom3 is required for apical constriction during lens pit morphogenesis. Development. 2014 Aug;141(16):3177-87
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7486722
Gene symbol: Shroom3
Gene name: shroom family member 3
Antibody: UPT132
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 5G: embryonic day 10.5; Ctnnd1tm1Abre/Ctnnd1tm1Abre (more )
Structure Level Pattern Image Note
TS17: lens placode Present 5G Localized mostly to the zonula adherens and did not appear to strongly colocalize with Ctnnd1 in prominent actomyosin filaments that spanned the apical cortex.
Specimen 5H: embryonic day 10.5; Ctnnd1tm1Abre/Ctnnd1tm1Abre, Tfap2atm1(cre)Moon/Tfap2a+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: lens pit Present 5H Quantifiably reduced in the apical junctions of hinge point cells, but apical localization in the central portion of the lens pit is unchanged.
Specimen 5I: embryonic day 10.5; Ctnnd1tm1Abre/Ctnnd1+, Shroom3Gt(ROSA53)Sor/Shroom3+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: lens pit Present 5I Significant reduction in both hinge point and central cells.
Specimen S3A: embryonic day 10.0 (more )
Note: Double labeled: red - Shroom3; green - Ctnnb1 (assay MGI:7486717).
Structure Level Pattern Image Note
TS16: lens placode Present S3A merge , S3A middle
Specimen S3C: embryonic day 10.0 (more )
Note: Double labeled: red - Shroom3; green - Myh10 (assay MGI:7486725).
Structure Level Pattern Image Note
TS16: lens placode Present S3C merge , S3C middle
Specimen S3D: embryonic day 10.0 (more )
Note: Double labeled: red - Shroom3; green - Ctnnd1 (assay MGI:7486721).
Structure Level Pattern Image Note
TS16: lens placode Present S3D merge , S3D middle
Specimen S3E/F: embryonic day 10.5 (more )
Note: Double labeled: red - Shroom3; green - Ctnnd1 (assay MGI:7486721).
Structure Level Pattern Image Note
TS17: lens pit Present S3F merge , S3F middle , S3E middle , S3E merge Localized mostly to the zonula adherens and did not appear to strongly colocalize with Ctnnd1 in prominent actomyosin filaments that spanned the apical cortex.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/10/2024
MGI 6.24 		The Jackson Laboratory