Results
|
Specimen
1B:
embryonic day 12.5; Foxb1tm1Sli/Foxb1 +
(more )
Specimen
1B:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1+
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
1C:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1tm1Sli
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
1E:
embryonic day 14.5; Foxb1tm1Sli/Foxb1 +
(more )
Specimen
1E:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
1F:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1tm1Sli
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
1H:
embryonic day 14.5; Foxb1tm1Sli/Foxb1 +
(more )
Specimen
1H:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
1I:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1tm1Sli
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
1J +/-:
embryonic day 10.5; Foxb1tm1Sli/Foxb1 +
(more )
Specimen
1J +/-:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1+
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: future spinal cord |
Present |
Widespread |
1J left
|
Expressed throughout the neuroepithelium.
|
TS17: brain |
Present |
Regionally restricted |
1J left
|
Expressed throughout the neuroepithelium, posterior to the future telencephalon.
|
Specimen
1J -/-:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1tm1Sli
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: future spinal cord |
Present |
Widespread |
1J right
|
Expressed throughout the neuroepithelium.
|
TS17: brain |
Present |
Regionally restricted |
1J right
|
Expressed throughout the neuroepithelium, posterior to the future telencephalon.
|
Specimen
1K +/-:
embryonic day 18.5; Foxb1tm1Sli/Foxb1 +
(more )
Specimen
1K +/-:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1+
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1K -/-:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1tm1Sli
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1L +/-:
embryonic day 18.5; Foxb1tm1Sli/Foxb1 +
(more )
Specimen
1L +/-:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1+
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1L -/-:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1tm1Sli
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: pretectum |
Present |
|
1L right
|
Smaller expression domain compared with the heterozygous.
|
Specimen
3A:
embryonic day 18.5; Foxb1tm1Sli/Foxb1 +
(more )
Specimen
3A:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1+
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: spinal cord ventral horn |
Present |
Regionally restricted |
3A
|
Expressed in the entire ventromedial spinal cord, and ventrolateral spinal cord at lumbar levels.
|
Specimen
3B:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1tm1Sli
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: spinal cord lateral wall |
Present |
Regionally restricted |
3B
|
Positive ventromedial columns were less prominent, and there was an increased expression throughout the spinal cord.
|
TS26: spinal cord ventral horn |
Present |
Regionally restricted |
3B
|
Positive ventromedial columns were less prominent.
|
Specimen
3C:
embryonic day 18.5; Foxb1tm1Sli/Foxb1 +
(more )
Note: Midthoracic level.
Specimen
3C:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1+
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Midthoracic level.
|
Specimen
3D:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1tm1Sli
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Midthoracic level.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: spinal cord lateral wall |
|
Present |
Regionally restricted |
3D
|
The decreased prominence (62%, summarized in Table 1) of the ventromedial columns was partly due to the dispersion of these cells, compared to their clustered arrangement in the heterozygote.
|
TS26: spinal cord thoracic region |
|
Present |
Regionally restricted |
3D
|
The decreased prominence (62%, summarized in Table 1) of the ventromedial columns was partly due to the dispersion of these cells, compared to their clustered arrangement in the heterozygote.
|
TS26: spinal cord dorsal horn |
glial cell |
Present |
Regionally restricted |
3D
|
Concentrated in the dorsal gray matter (summarized in Table 1).
|
TS26: spinal cord ventral horn |
|
Present |
|
3D
|
Numerous small positive cells were also found (summarized in Table 1).
|
TS26: spinal cord white matter |
|
Present |
|
3D
|
Numerous small positive cells were also found (summarized in Table 1).
|
Specimen
3E:
embryonic day 18.5; Foxb1tm1Sli/Foxb1 +
(more )
Note: Lumbar 1-2 level.
Specimen
3E:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1+
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Lumbar 1-2 level.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: spinal cord lateral wall |
|
Present |
Regionally restricted |
3E
|
Positive cells were concentrated in the most ventral portion of the ventral horn, with large, ovoid nuclei. Other positive cells with small narrow nuclei were found in the dorsal spinal cord.
|
TS26: spinal cord lumbar region |
|
Present |
Regionally restricted |
3E
|
Positive cells were concentrated in the most ventral portion of the ventral horn, with large, ovoid nuclei. Other positive cells with small narrow nuclei were found in the dorsal spinal cord.
|
TS26: spinal cord lumen |
ependymal cell |
Present |
|
3E
|
|
TS26: spinal cord ventral horn |
|
Present |
Regionally restricted |
3E
|
Positive cells were concentrated in the most ventral portion.
|
TS26: spinal cord dorsal horn |
|
Present |
Regionally restricted |
3E
|
Positive cells with small narrow nuclei were found in the dorsal spinal cord.
|
Specimen
3F:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1tm1Sli
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Lumbar 1-2 level.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: spinal cord lateral wall |
spinal cord motor neuron |
Present |
Regionally restricted |
3F
|
Similar as in the heterozygous (summarized in Table 1). Expressed in the lateral motor column.
|
TS26: spinal cord lumbar region |
spinal cord motor neuron |
Present |
Regionally restricted |
3F
|
Similar as in the heterozygous (summarized in Table 1). Expressed in the lateral motor column.
|
TS26: spinal cord dorsal horn |
glial cell |
Present |
Regionally restricted |
3F
|
Concentrated in the dorsal gray matter, similar pattern as in the heterozygous, with an increased number and broader distribution (summarized in Table 1).
|
TS26: spinal cord ventral horn |
|
Present |
|
3F
|
Numerous small positive cells were also found.
|
TS26: spinal cord white matter |
|
Present |
|
3F
|
Numerous small positive cells were also found.
|
Specimen
3G:
embryonic day 18.5; Foxb1tm1Sli/Foxb1 +
(more )
Note: Thoracic level. Double labeled: blue - Foxb1 reporter; brown - acetylcholinesterase activity staining.
Specimen
3G:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1+
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Thoracic level. Double labeled: blue - Foxb1 reporter; brown - acetylcholinesterase activity staining.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: spinal cord lateral wall |
spinal cord motor neuron |
Present |
Regionally restricted |
3G
|
Positive cells were concentrated in the most ventral portion of the ventral horn, with large, ovoid nuclei (identified as neurons by acetylcholinesterase activity). Only a subset of the ventromedial LacZ+ neurons were motor neurons and appeared to form a subset of the medial motor column.
|
TS26: spinal cord thoracic region |
spinal cord motor neuron |
Present |
Regionally restricted |
3G
|
Positive cells were concentrated in the most ventral portion of the ventral horn, with large, ovoid nuclei (identified as neurons by acetylcholinesterase activity). Only a subset of the ventromedial LacZ+ neurons were motor neurons and appeared to form a subset of the medial motor column.
|
TS26: spinal cord ventral horn |
glial cell |
Present |
Regionally restricted |
3G
|
Only a few LacZ positive glial cells (small narrow nuclei) were found in the ventral spinal cord in the heterozygous mutant.
|
TS26: spinal cord dorsal horn |
spinal cord ventral column interneuron |
Present |
Regionally restricted |
3G
|
LacZ positive without acetylcholinesterase activity.
|
Specimen
3H:
embryonic day 18.5; Foxb1tm1Sli/ Foxb1tm1Sli
(more )
Note: Thoracic level. Double labeled: blue - Foxb1 reporter; brown - acetylcholinesterase activity staining.
Specimen
3H:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1tm1Sli
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Thoracic level. Double labeled: blue - Foxb1 reporter; brown - acetylcholinesterase activity staining.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: spinal cord thoracic region |
spinal cord motor neuron |
Present |
Regionally restricted |
3H
|
Positive cells were concentrated in the most ventral portion of the ventral horn, with large, ovoid nuclei (identified as neurons by acetylcholinesterase activity).
|
TS26: spinal cord ventral horn |
spinal cord motor neuron |
Present |
Regionally restricted |
3H
|
Positive cells were concentrated in the most ventral portion of the ventral horn, with large, ovoid nuclei (identified as neurons by acetylcholinesterase activity).
|
TS26: spinal cord lateral wall |
glial cell |
Present |
Regionally restricted |
3H
|
Many LacZ positive glial cells (small narrow nuclei) were found in the homozygous mutant.
|
TS26: spinal cord lateral wall |
spinal cord ventral column interneuron |
Present |
Scattered |
3H
|
LacZ positive without acetylcholinesterase activity. A 44% reduction of positive thoracic interneurons compared with the heterozygous (summarized in Table 1).
|
TS26: spinal cord ventral horn |
|
Present |
|
3H
|
Numerous small positive cells were also found.
|
TS26: spinal cord white matter |
|
Present |
|
3H
|
Numerous small positive cells were also found.
|
Specimen
4A:
embryonic day 13.5; Foxb1tm1Sli/Foxb1 +
(more )
Note: Cervical level. Double labeled: blue - Foxb1 reporter; brown - Isl1 (assay MGI:7488703) and Isl2 (assay MGI:7490072).
Specimen
4A:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Cervical level. Double labeled: blue - Foxb1 reporter; brown - Isl1 (assay MGI:7488703) and Isl2 (assay MGI:7490072).
|
Specimen
4B:
embryonic day 13.5; Foxb1tm1Sli/Foxb1 +
(more )
Note: Thoracic level. Double labeled: blue - Foxb1 reporter; brown - Isl1 (assay MGI:7488703) and Isl2 (assay MGI:7490072).
Specimen
4B:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Thoracic level. Double labeled: blue - Foxb1 reporter; brown - Isl1 (assay MGI:7488703) and Isl2 (assay MGI:7490072).
|
Specimen
4C:
embryonic day 13.5; Foxb1tm1Sli/Foxb1 +
(more )
Note: Upper lumbar level. Double labeled: blue - Foxb1 reporter; brown - Isl1 (assay MGI:7488703) and Isl2 (assay MGI:7490072).
Specimen
4C:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Upper lumbar level. Double labeled: blue - Foxb1 reporter; brown - Isl1 (assay MGI:7488703) and Isl2 (assay MGI:7490072).
|
Specimen
4D:
embryonic day 13.5; Foxb1tm1Sli/Foxb1 +
(more )
Note: Mid lumbar level. Double labeled: blue - Foxb1 reporter; brown - Isl1 (assay MGI:7488703) and Isl2 (assay MGI:7490072).
Specimen
4D:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Mid lumbar level. Double labeled: blue - Foxb1 reporter; brown - Isl1 (assay MGI:7488703) and Isl2 (assay MGI:7490072).
|
Specimen
4E:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1tm1Sli
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Cervical level. Double labeled: blue - Foxb1 reporter; brown - Isl1 (assay MGI:7488703) and Isl2 (assay MGI:7490072).
|
Specimen
4F:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1tm1Sli
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Thoracic level. Double labeled: blue - Foxb1 reporter; brown - Isl1 (assay MGI:7488703) and Isl2 (assay MGI:7490072).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS21: spinal cord thoracic region |
spinal cord motor neuron |
Present |
Regionally restricted |
4F
|
Expressed in a subset of cells (medial half) in the medial motor column. A 49% decrease in thoracic positive cells compared with the heterozygous (summarized in Table 1).
|
TS21: spinal cord ventral horn |
spinal cord motor neuron |
Present |
Regionally restricted |
4F
|
Expressed in a subset of cells (medial half) in the medial motor column. A 49% decrease in thoracic positive cells compared with the heterozygous (summarized in Table 1).
|
TS21: spinal cord thoracic region |
spinal cord ventral column interneuron |
Present |
Regionally restricted |
4F
|
A 42% increase in the thoracic positive interneuron population compared with the heterozygous (summarized in Table 1).
|
TS21: spinal cord ventral horn |
spinal cord ventral column interneuron |
Present |
Regionally restricted |
4F
|
A 42% increase in the thoracic positive interneuron population compared with the heterozygous (summarized in Table 1).
|
Specimen
4G:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1tm1Sli
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Upper lumbar level. Double labeled: blue - Foxb1 reporter; brown - Isl1 (assay MGI:7488703) and Isl2 (assay MGI:7490072).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS21: spinal cord lumbar region |
spinal cord motor neuron |
Present |
Regionally restricted |
4G
|
Similar as in the heterozygous. Expressed in the lateral half of lateral motor column. A 52% increase in lumbar positive cells (summarized in Table 1) compared with the heterozygous.
|
TS21: spinal cord ventral horn |
spinal cord motor neuron |
Present |
Regionally restricted |
4G
|
Similar as in the heterozygous. Expressed in the lateral half of lateral motor column. A 52% increase in lumbar positive cells (summarized in Table 1) compared with the heterozygous.
|
TS21: spinal cord lumbar region |
spinal cord ventral column interneuron |
Present |
|
4G
|
Increased by 93% compared to the heterozygous (summarized in Table 1).
|
TS21: spinal cord ventral horn |
spinal cord ventral column interneuron |
Present |
|
4G
|
Increased by 93% compared to the heterozygous (summarized in Table 1).
|
Specimen
4H:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1tm1Sli
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Mid lumbar level. Double labeled: blue - Foxb1 reporter; brown - Isl1 (assay MGI:7488703) and Isl2 (assay MGI:7490072).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS21: spinal cord lumbar region |
|
Present |
Regionally restricted |
4H
|
Similar as in the heterozygous. Expressed in the lateral half of lateral motor column. A 52% increase in lumbar positive cells (summarized in Table 1) compared with the heterozygous.
|
TS21: spinal cord ventral horn |
|
Present |
Regionally restricted |
4H
|
Similar as in the heterozygous. Expressed in the lateral half of lateral motor column. A 52% increase in lumbar positive cells (summarized in Table 1) compared with the heterozygous.
|
TS21: spinal cord lumbar region |
spinal cord ventral column interneuron |
Present |
|
4H
|
Increased by 93% compared to the heterozygous (summarized in Table 1).
|
TS21: spinal cord ventral horn |
spinal cord ventral column interneuron |
Present |
|
4H
|
Increased by 93% compared to the heterozygous (summarized in Table 1).
|
Specimen
not shown:
embryonic day 14.5; Foxb1tm1Sli/Foxb1 +
(more )
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
not shown:
embryonic day 18.5; Foxb1tm1Sli/Foxb1 +
(more )
Note: Double labeled: blue - Foxb1 reporter; brown - Gfap (assay MGI:7489616).
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1+
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Foxb1 reporter; brown - Gfap (assay MGI:7489616).
|
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1tm1Sli
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
not shown:
embryonic day 18.5; Foxb1tm1Sli/Foxb1 +
(more )
Note: Double labeled: blue - Foxb1 reporter; brown - Nes (assay MGI:7489617).
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1+
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Foxb1 reporter; brown - Nes (assay MGI:7489617).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: spinal cord lateral wall |
glial cell |
Present |
Regionally restricted |
|
Coexpressed with Nes in a subset of cells with small narrow nuclei. LacZ positive cells in the medial and lateral ventral columns did not express glial marker Nes.
|
Specimen
not shown:
embryonic day 10.5; Foxb1tm1Sli/Foxb1 +
(more )
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1+
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS17: future spinal cord |
spinal cord motor neuron |
Present |
|
|
Positive newly generated motor neurons were seen.
|
Specimen
not shown:
embryonic day 11.5; Foxb1tm1Sli/Foxb1 +
(more )
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1+
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
spinal cord motor neuron |
Present |
|
|
Positive newly generated motor neurons were seen.
|
Specimen
not shown:
embryonic day 12.5; Foxb1tm1Sli/Foxb1 +
(more )
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1+
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS20: spinal cord ventral horn |
spinal cord ventral column interneuron |
Present |
|
|
Positive newly generated interneurons were seen.
|
Specimen
not shown:
embryonic day 13.5; Foxb1tm1Sli/Foxb1 +
(more )
Note: Sacral level.
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6
|
Mutant Allele(s):
|
Foxb1tm1Sli/Foxb1+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Sacral level.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS21: spinal cord ventral horn |
spinal cord motor neuron |
Present |
Regionally restricted |
|
Expressed in a subset of cells in the lateral motor column. Very few positive cells were found in the medial motor column at this sacral level.
|
|