Gene Expression Data
Assay Details
Assay
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Reference:
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J:244948
Wang DY, et al., Endoplasmic reticulum resident protein 44 (ERp44) deficiency in mice and zebrafish leads to cardiac developmental and functional defects. J Am Heart Assoc. 2014 Oct 20;3(5):e001018
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7493443
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Gene symbol:
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Erp44
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Gene name:
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endoplasmic reticulum protein 44
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Results
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Specimen
3A +/+:
postnatal adult
(more )
Specimen
3A +/+:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3A -/-:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Erp44Gt(XB599)Byg/Erp44Gt(XB599)Byg
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4D ERp44 E13:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Erp44Gt(XB599)Byg/Erp44+
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS21: heart |
Present |
Regionally restricted |
4D ERp44 E13
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Authors report a very specific expression pattern during maturation of heart.
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Specimen
4D ERp44 P1:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Erp44Gt(XB599)Byg/Erp44+
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS27: heart |
Present |
Regionally restricted |
4D ERp44 P1
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Authors report a very specific expression pattern during maturation of heart.
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Specimen
4D ERp44 P7:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Erp44Gt(XB599)Byg/Erp44+
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: heart |
Present |
Regionally restricted |
4D ERp44 P7
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Authors report a very specific expression pattern during maturation of heart.
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Specimen
4D ERp44 12 wk:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Erp44Gt(XB599)Byg/Erp44+
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Age:
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postnatal week 12
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: heart |
Present |
Regionally restricted |
4D ERp44 12 w
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Authors report a very specific expression pattern during maturation of heart.
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Specimen
4E ERp44:
postnatal day 7; Erp44Gt(XB599)Byg/Erp44 +
(more )
Note: Double labeled: green - Erp44 protein; red - Erp44 reporter (assay MGI:7493439).
Specimen
4E ERp44:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Erp44Gt(XB599)Byg/Erp44+
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Erp44 protein; red - Erp44 reporter (assay MGI:7493439).
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Specimen
Epicardium +/+ not shown:
postnatal week 12
(more )
Specimen
Epicardium +/+ not shown:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal week 12
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
Epicardium +/- not shown:
postnatal week 12; Erp44Gt(XB599)Byg/Erp44 +
(more )
Specimen
Epicardium +/- not shown:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd
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Mutant Allele(s):
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Erp44Gt(XB599)Byg/Erp44+
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Age:
|
postnatal week 12
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: visceral pericardium |
Weak |
|
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Expression in epicardium signficantly lower in heterozygote compared to wild type.
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Specimen
Myocardium +/+ not shown:
postnatal week 12
(more )
Specimen
Myocardium +/+ not shown:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal week 12
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
Myocardium +/- not shown:
postnatal week 12; Erp44Gt(XB599)Byg/Erp44 +
(more )
Specimen
Myocardium +/- not shown:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Erp44Gt(XB599)Byg/Erp44+
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Age:
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postnatal week 12
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4G:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Erp44Gt(XB599)Byg/Erp44+
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: heart |
Very strong |
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4G
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Very strong expression at E13.
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Specimen
4H:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Erp44Gt(XB599)Byg/Erp44+
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Age:
|
embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
|
Sex:
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Not Specified
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Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: heart |
Very strong |
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4H
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Very strong expression at E13.
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Specimen
4I:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Erp44Gt(XB599)Byg/Erp44+
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Age:
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postnatal week 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: heart |
Present |
Regionally restricted |
4I
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High expression in outer epicardial region and epicardial/myocardial border.
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Specimen
4J:
postnatal adult; Erp44Gt(XB599)Byg/Erp44 +
(more )
Note: Multi-labeled: green - Erp44; white - Pecam1 (assay MGI:7493445); red - phalloidin (F-actin); blue - Hoechst.
Specimen
4J:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Erp44Gt(XB599)Byg/Erp44+
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Age:
|
postnatal adult
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
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Multi-labeled: green - Erp44; white - Pecam1 (assay MGI:7493445); red - phalloidin (F-actin); blue - Hoechst.
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Specimen
4K ERp44:
(close )
Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
|
Erp44Gt(XB599)Byg/Erp44+
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Age:
|
postnatal adult
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: heart |
Present |
Graded |
4K ERp44
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Graded expression in epicardium/myocardium region
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