Gene Expression Data
Assay Details
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Assay
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Reference:
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J:296375
Holmes G, et al., Integrated Transcriptome and Network Analysis Reveals Spatiotemporal Dynamics of Calvarial Suturogenesis. Cell Rep. 2020 Jul 7;32(1):107871
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7494355
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Gene symbol:
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Top2a
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Gene name:
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topoisomerase (DNA) II alpha
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Probe:
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Mm-Top2a (491221)
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Probe preparation:
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Antisense
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Visualized with:
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Fluorescence
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Assay notes:
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Assay is smFISH using RNAscope probe.
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Results
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Specimen
3F FS4 Top2a:
embryonic day 18.5
(more  )
Specimen
3F FS4 Top2a:
(close  )
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Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: frontal bone |
Present |
Regionally restricted |
3F FS4 Top2a
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Strongest expression at edge of frontal bones, marking presumptive osteogenic fronts.
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Analysis Tools
