Probe:	Tbx20 probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Cell Type	Level	Pattern	Image	Note
TS20: hindbrain lateral wall	branchiomotor neuron	Present	Regionally restricted	2E	Expressed in areas spanning r4 to r6.
TS20: hindbrain lateral wall	trigeminal motor neuron	Present	Regionally restricted	2E	Expressed in trigeminal motor neurons (asterisk).

Structure	Level	Pattern	Image	Note
TS20: hindbrain lateral wall	Present	Regionally restricted	2F	All neurons migrated caudally into r5 and r6.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: hindbrain lateral wall	branchiomotor neuron	Present	Regionally restricted	2G	A large number of neurons migrated rostrally into r3 and r2 (arrowheads).

Structure	Cell Type	Level	Pattern	Image	Note
TS20: hindbrain lateral wall	branchiomotor neuron	Present	Regionally restricted	2H	Rostral inappropriate migration was almost completely suppressed in double mutants.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: hindbrain lateral wall	branchiomotor neuron	Present	Regionally restricted	3D	Expressed in rhombomere 4 (r4) to r6.
TS20: hindbrain lateral wall	trigeminal motor neuron	Present	Regionally restricted	3D	Expressed in rhombomere 2 (asterisks).

Structure	Cell Type	Level	Pattern	Image	Note
TS20: hindbrain lateral wall	branchiomotor neuron	Present	Regionally restricted	3E	Expressed in rhombomere 4 (r4) to r6, similar as in the wild-type (quantified in Fig 3J).
TS20: hindbrain lateral wall	trigeminal motor neuron	Present	Regionally restricted	3E	Expressed in rhombomere 2 (asterisks).

Structure	Cell Type	Level	Pattern	Image	Note
TS20: hindbrain lateral wall	branchiomotor neuron	Present	Regionally restricted	3F	Dramatic increase in rostral migration. Neurons migrated rostrally into r2 and beyond (quantified in Fig 3J, S7C).
TS20: hindbrain lateral wall	trigeminal motor neuron	Present	Regionally restricted	3F	Expressed in rhombomere 2 (asterisks), caudal edge marking the r2-r3 boundary.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: hindbrain lateral wall	branchiomotor neuron	Present	Regionally restricted	3G	A significant number of positive neurons were located in r3 (quantified in Fig 3J, S7A).
TS20: hindbrain lateral wall	trigeminal motor neuron	Present	Regionally restricted	3G	Expressed in rhombomere 2 (asterisks), caudal edge marking the r2-r3 boundary.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: hindbrain lateral wall	branchiomotor neuron	Present	Regionally restricted	3H	A large number of positive neurons were located in r3 (quantified in Fig 3J, S7B), with neurons spilling over into r2.
TS20: hindbrain lateral wall	trigeminal motor neuron	Present	Regionally restricted	3H	Expressed in rhombomere 2 (asterisks).

Structure	Cell Type	Level	Pattern	Image	Note
TS20: hindbrain lateral wall	branchiomotor neuron	Present	Regionally restricted	3I	Neurons migrated extensively into r3. Although more numerous, appeared to remain largely within r3 (quantified in Fig 3J, S7D). There was a marked reduction in the size of the caudal migratory stream.
TS20: hindbrain lateral wall	trigeminal motor neuron	Present	Regionally restricted	3I	Expressed in rhombomere 2 (asterisks), caudal edge marking the r2-r3 boundary.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: hindbrain lateral wall	branchiomotor neuron	Present	Regionally restricted	S3A	Expressed in rhombomere 4 (r4) to r6.
TS20: hindbrain lateral wall	trigeminal motor neuron	Present	Regionally restricted	S3A	Expressed in rhombomere 2 (asterisks).

Structure	Cell Type	Level	Pattern	Image	Note
TS20: hindbrain lateral wall	trigeminal motor neuron	Present	Regionally restricted	S3B	Expressed in rhombomere 2 (asterisks), similar as in the wild-type.
TS20: hindbrain lateral wall	branchiomotor neuron	Present	Regionally restricted	S3B	Expressed mainly in rhombomere 4 (r4) to r6. Slightly migration into r3 was seen.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: hindbrain lateral wall	branchiomotor neuron	Present	Regionally restricted	S3C	Neurons migrated rostrally into r3 and r2.
TS20: hindbrain lateral wall	trigeminal motor neuron	Present	Regionally restricted	S3C	Expressed in rhombomere 2 (asterisks), similar as in the wild-type.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: hindbrain lateral wall	trigeminal motor neuron	Present	Regionally restricted	S3D	Expressed in rhombomere 2 (asterisks).
TS20: hindbrain lateral wall	branchiomotor neuron	Present	Regionally restricted	S3D	Expressed in rhombomere 4 (r4) to r6.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: hindbrain lateral wall	trigeminal motor neuron	Present	Regionally restricted	S3E	Expressed in rhombomere 2 (asterisks), similar as in the wild-type.
TS20: hindbrain lateral wall	branchiomotor neuron	Present	Regionally restricted	S3E	Expressed in rhombomere 4 (r4) to r6, similar as in the wild-type.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: hindbrain lateral wall	trigeminal motor neuron	Present	Regionally restricted	S3F	Expressed in rhombomere 2 (asterisks), similar as in the wild-type.
TS20: hindbrain lateral wall	branchiomotor neuron	Present	Regionally restricted	S3F	Expressed in rhombomere 4 (r4) to r6, similar as in the wild-type.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: hindbrain lateral wall	trigeminal motor neuron	Present	Regionally restricted	S6A	Expressed in rhombomere 2 (asterisks), caudal edge marking the r2-r3 boundary.
TS20: hindbrain lateral wall	branchiomotor neuron	Present	Regionally restricted	S6A	Expressed in rhombomere 4 (r4) to r6.