Myo7a Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:276652 Fang Q, et al., Deletion of Limk1 and Limk2 in mice does not alter cochlear development or auditory function. Sci Rep. 2019 Mar 4;9(1):3357
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7506974
Gene symbol:	Myo7a
Gene name:	myosin VIIA

Antibody:	anti-myosin VIIa (#25-6790)
Assay notes:	Fixation with 4% polyoxymethylene.

Results

1a Myo7a P21 (left) ©

1a P21 Merged (left) ©

1a P21 z-stack (left) ©

Specimen 1a Myo7a P21 (left): postnatal day 21 (more

)
Note: Double labeled: green - Myo7a; red - Limk1 (assay MGI:7506975).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		1a Myo7a P21 (left) , 1a P21 z-stack (left) , 1a P21 Merged (left)

1a Myo7a P30 (left) ©

1a P30 Merged (left) ©

1a P30 z-stack (left) ©

Specimen 1a Myo7a P30 (left): postnatal day 30 (more

)
Note: Double labeled: green - Myo7a; red - Limk1 (assay MGI:7506975).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		1a Myo7a P30 (left) , 1a P30 Merged (left) , 1a P30 z-stack (left)

1a Myo7a P21 (right) ©

1a P21 Merged (right) ©

1a P21 z-stack (right) ©

Specimen 1a Myo7a P21 (right): postnatal day 21 (more

)
Note: Double labeled: green - Myo7a; red - Limk2 (assay MGI:7506977).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		1a P21 Merged (right) , 1a Myo7a P21 (right) , 1a P21 z-stack (right)

1a Myo7a P30 (right) ©

1a P30 Merged (right) ©

1a P30 z-stack (right) ©

Specimen 1a Myo7a P30 (right): postnatal day 30 (more

)
Note: Double labeled: green - Myo7a; red - Limk2 (assay MGI:7506977).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		1a P30 Merged (right) , 1a P30 z-stack (right) , 1a Myo7a P30 (right)

2a Cytoplasm WT Merged (left) ©

2a Cytoplasm WT Myo7a (left) ©

Specimen 2a Myo7a Cytoplasm WT (left): postnatal day 3 (more

)
Note: Double labeled: green - Myo7a; red - Limk1 (assay MGI:7506975).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlea	cochlea auditory hair cell	Present		2a Cytoplasm WT Myo7a (left) , 2a Cytoplasm WT Merged (left)

2a Cytoplasm DKO Merged (left) ©

2a Cytoplasm DKO Myo7a (left) ©

Specimen 2a Myo7a Cytoplasm DKO (left): postnatal day 3; Limk1^tm1Zpj/Limk1^tm1Zpj, Limk2^tm1.1Tnak/Limk2^tm1.1Tnak (more

)
Note: Double labeled: green - Myo7a; red - Limk1 (assay MGI:7506975).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlea	cochlea auditory hair cell	Present		2a Cytoplasm DKO Myo7a (left) , 2a Cytoplasm DKO Merged (left)

2a Cytoplasm WT Merged (right) ©

2a Cytoplasm WT Myo7a (right) ©

Specimen 2a Myo7a Cytoplasm WT (right): postnatal day 3 (more

)
Note: Double labeled: green - Myo7a; red - Limk2 (assay MGI:7506977).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlea	cochlea auditory hair cell	Present		2a Cytoplasm WT Myo7a (right) , 2a Cytoplasm WT Merged (right)

2a Cytoplasm DKO Merged (right) ©

2a Cytoplasm DKO Myo7a (right) ©

Specimen 2a Myo7a Cytoplasm DKO (right): postnatal day 3; Limk1^tm1Zpj/Limk1^tm1Zpj, Limk2^tm1.1Tnak/Limk2^tm1.1Tnak (more

)
Note: Double labeled: green - Myo7a; red - Limk2 (assay MGI:7506977).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlea	cochlea auditory hair cell	Present		2a Cytoplasm DKO Merged (right) , 2a Cytoplasm DKO Myo7a (right)

2a Nucleus WT Merged (left) ©

2a Nucleus WT Myo7a (left) ©

Specimen 2a Myo7a Nucleus WT (left): postnatal day 3 (more

)
Note: Double labeled: green - Myo7a; red - Limk1 (assay MGI:7506975).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlea	cochlea auditory hair cell	Present		2a Nucleus WT Merged (left) , 2a Nucleus WT Myo7a (left)

2a Nucleus DKO Merged (left) ©

2a Nucleus DKO Myo7a (left) ©

Specimen 2a Myo7a Nucleus DKO (left): postnatal day 3; Limk1^tm1Zpj/Limk1^tm1Zpj, Limk2^tm1.1Tnak/Limk2^tm1.1Tnak (more

)
Note: Double labeled: green - Myo7a; red - Limk1 (assay MGI:7506975).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlea	cochlea auditory hair cell	Present		2a Nucleus DKO Merged (left) , 2a Nucleus DKO Myo7a (left)

2a Nucleus WT Merged (right) ©

2a Nucleus WT Myo7a (right) ©

Specimen 2a Myo7a Nucleus WT (right): postnatal day 3 (more

)
Note: Double labeled: green - Myo7a; red - Limk2 (assay MGI:7506977).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlea	cochlea auditory hair cell	Present		2a Nucleus WT Myo7a (right) , 2a Nucleus WT Merged (right)

2a Nucleus DKO Merged (right) ©

2a Nucleus DKO Myo7a (right) ©

Specimen 2a Myo7a Nucleus DKO (right): postnatal day 3; Limk1^tm1Zpj/Limk1^tm1Zpj, Limk2^tm1.1Tnak/Limk2^tm1.1Tnak (more

)
Note: Double labeled: green - Myo7a; red - Limk2 (assay MGI:7506977).

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlea	cochlea auditory hair cell	Present		2a Nucleus DKO Merged (right) , 2a Nucleus DKO Myo7a (right)

3a P7 WT ©

Specimen 3a P7 WT: postnatal day 7 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		3a P7 WT

3a P7 DKO ©

Specimen 3a P7 DKO: postnatal day 7; Limk1^tm1Zpj/Limk1^tm1Zpj, Limk2^tm1.1Tnak/Limk2^tm1.1Tnak (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		3a P7 DKO	No difference in expression from apical to basal turns.

3a P30 WT ©

Specimen 3a P30 WT: postnatal day 30 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		3a P30 WT

3a P30 DKO ©

Specimen 3a P30 DKO: postnatal day 30; Limk1^tm1Zpj/Limk1^tm1Zpj, Limk2^tm1.1Tnak/Limk2^tm1.1Tnak (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		3a P30 DKO	No difference in expression from apical to basal turns.

3a P120 WT ©

Specimen 3a P120 WT: postnatal day 120 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		3a P120 WT

3a P120 DKO ©

Specimen 3a P120 DKO: postnatal day 120; Limk1^tm1Zpj/Limk1^tm1Zpj, Limk2^tm1.1Tnak/Limk2^tm1.1Tnak (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		3a P120 DKO	No difference in expression from apical to basal turns.

S3_APEX P7 (upper) ©

Specimen S3 APEX P7 (upper): postnatal day 7 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		S3_APEX P7 (upper)

S3_APEX P7 (lower) ©

Specimen S3 APEX P7 (lower): postnatal day 7; Limk1^tm1Zpj/Limk1^tm1Zpj, Limk2^tm1.1Tnak/Limk2^tm1.1Tnak (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		S3_APEX P7 (lower)	Hair cells were morphologically normal.

S3_APEX P30 (upper) ©

Specimen S3 APEX P30 (upper): postnatal day 30 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		S3_APEX P30 (upper)

S3_APEX P30 (lower) ©

Specimen S3 APEX P30 (lower): postnatal day 30; Limk1^tm1Zpj/Limk1^tm1Zpj, Limk2^tm1.1Tnak/Limk2^tm1.1Tnak (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		S3_APEX P30 (lower)	Hair cells were morphologically normal.

S3_APEX P120 (upper) ©

Specimen S3 APEX P120 (upper): postnatal day 120 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		S3_APEX P120 (upper)

S3_APEX P120 (lower) ©

Specimen S3 APEX P120 (lower): postnatal day 120; Limk1^tm1Zpj/Limk1^tm1Zpj, Limk2^tm1.1Tnak/Limk2^tm1.1Tnak (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		S3_APEX P120 (lower)	Hair cells were morphologically normal.

S3_MID P7 (upper) ©

Specimen S3 MID P7 (upper): postnatal day 7 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		S3_MID P7 (upper)

S3_MID P7 (lower) ©

Specimen S3 MID P7 (lower): postnatal day 7; Limk1^tm1Zpj/Limk1^tm1Zpj, Limk2^tm1.1Tnak/Limk2^tm1.1Tnak (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		S3_MID P7 (lower)	Hair cells were morphologically normal.

S3_MID P30 (upper) ©

Specimen S3 MID P30 (upper): postnatal day 30 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		S3_MID P30 (upper)

Specimen S3 MID P30 (lower): postnatal day 30; Limk1^tm1Zpj/Limk1^tm1Zpj, Limk2^tm1.1Tnak/Limk2^tm1.1Tnak (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		S3_MID P30 (lower)	Hair cells were morphologically normal.

Specimen S3 MID P120 (upper): postnatal day 120 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		S3_MID P120 (upper)

Specimen S3 MID P120 (lower): postnatal day 120; Limk1^tm1Zpj/Limk1^tm1Zpj, Limk2^tm1.1Tnak/Limk2^tm1.1Tnak (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		S3_MID P120 (lower)	Hair cells were morphologically normal.

Specimen S5a P0 WT: postnatal day 0 (more

)
Note: Double labeled: red - Myo7a; green - FM143.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlea	cochlea auditory hair cell	Present		S5a P0 WT Merged , S5a P0 WT Myo7a

Specimen S5a P0 DKO: postnatal day 0; Limk1^tm1Zpj/Limk1^tm1Zpj, Limk2^tm1.1Tnak/Limk2^tm1.1Tnak (more

)
Note: Double labeled: red - Myo7a; green - FM143.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlea	cochlea auditory hair cell	Present		S5a P0 DKO Myo7a , S5a P0 DK0 Merged	No difference in expression from apical to basal turns.

Specimen S5a P14 WT: postnatal day 14 (more

)
Note: Double labeled: red - Myo7a; green - FM143.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		S5a P14 WT Myo7a , S5a P14 WT Merged

Specimen S5a P14 DKO: postnatal day 14; Limk1^tm1Zpj/Limk1^tm1Zpj, Limk2^tm1.1Tnak/Limk2^tm1.1Tnak (more

)
Note: Double labeled: red - Myo7a; green - FM143.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		S5a P14 DKO Myo7a , S5a P14 DKO Merged	No difference in expression from apical to basal turns.

Specimen 6c Myo7a WT (upper): postnatal day 30 (more

)
Note: Double labeled: green - Myo7a; red - Cfl1 (assay MGI:7506984).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		6c Merged WT (upper) , 6c Myo7a WT (upper)

Specimen 6c Myo7a DKO (upper): postnatal day 30; Limk1^tm1Zpj/Limk1^tm1Zpj, Limk2^tm1.1Tnak/Limk2^tm1.1Tnak (more

)
Note: Double labeled: green - Myo7a; red - Cfl1 (assay MGI:7506984).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		6c Merged DKO (upper) , 6c Myo7a DKO (upper)

Specimen 6c Myo7a WT (lower): postnatal day 30 (more

)
Note: Double labeled: green - Myo7a; red - Cfl1 (p-cofilin assay MGI:7506985).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		6c Merged WT (lower) , 6c Myo7a WT (lower)

Specimen 6c Myo7a DKO (lower): postnatal day 30; Limk1^tm1Zpj/Limk1^tm1Zpj, Limk2^tm1.1Tnak/Limk2^tm1.1Tnak (more

)
Note: Double labeled: green - Myo7a; red - Cfl1 (p-cofilin assay MGI:7506985).

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cochlea	cochlea auditory hair cell	Present		6c Merged DKO (lower) , 6c Myo7a DKO (lower)

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