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Gene Expression Data
Assay Details
Assay
Reference: J:276652 Fang Q, et al., Deletion of Limk1 and Limk2 in mice does not alter cochlear development or auditory function. Sci Rep. 2019 Mar 4;9(1):3357
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7506978
Gene symbol: Sox2
Gene name: SRY (sex determining region Y)-box 2
Antibody: Anti-Sox2 (Y-17; sc-17320)
Assay notes: Fixation with 4% polyoxymethylene. Two Sox2 antibodies were described, but the one used in this assay was not detailed. Anti-Sox2 MGI:6863996 may have been used in this assay.
Results
Specimen S2 Sox2 P21 (left): postnatal day 21 (more )
Note: Double labeled: blue - Sox2; red - Limk1 (assay MGI:7506975).
Structure Cell Type Level Pattern Image Note
TS28: cochlea supporting cell of cochlea Present S2_P21 z-stack (left) , S2_Sox2 P21 (left) , S2_P21 Merged (left)
Specimen S2 Sox2 P30 (left): postnatal day 30 (more )
Note: Double labeled: blue - Sox2; red - Limk1 (assay MGI:7506975).
Structure Cell Type Level Pattern Image Note
TS28: cochlea supporting cell of cochlea Present S2_P30 Merged (left) , S2_Sox2 P30 (left) , S2_P30 z-stack (left)
Specimen S2 Sox2 P21 (right): postnatal day 21 (more )
Note: Double labeled: blue - Sox2; red - Limk2 (assay MGI:7506977).
Structure Cell Type Level Pattern Image Note
TS28: cochlea supporting cell of cochlea Present S2_P21 z-stack (right) , S2_Sox2 P21 (right) , S2_P21 Merged (right)
Specimen S2 Sox2 P30 (right): postnatal day 30 (more )
Note: Double labeled: blue - Sox2; red - Limk2 (assay MGI:7506977).
Structure Cell Type Level Pattern Image Note
TS28: cochlea supporting cell of cochlea Present S2_P30 z-stack (right) , S2_Sox2 P30 (right) , S2_P30 Merged (right)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/17/2024
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