Gene Expression Data
Assay Details
Assay
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Reference:
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J:84706
Assimacopoulos S, et al., Identification of a Pax6-dependent epidermal growth factor family signaling source at the lateral edge of the embryonic cerebral cortex. J Neurosci. 2003 Jul 23;23(16):6399-403
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7518932
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Gene symbol:
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Tgfa
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Gene name:
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transforming growth factor alpha
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Probe:
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TGF-alpha-cDNA6
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2A:
embryonic day 12.5
(more )
Specimen
2A:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Structure |
Level |
Pattern |
Image |
Note |
TS20: cortical antihem |
Strong |
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2A
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Curve of strong expression in viewed from lateral face of cortical primordium.
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Specimen
2F:
embryonic day 18.5
(more )
Specimen
2F:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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whole mount
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Structure |
Level |
Pattern |
Image |
Note |
TS26: cortical antihem |
Present |
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2F
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Expression maintained for several days in this position.
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Specimen
3B:
embryonic day 12.5
(more )
Specimen
3B:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebral cortex |
Present |
Regionally restricted |
3B
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Expression domain lies within cortical primordium.
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Specimen
3D:
embryonic day 12.5
(more )
Note: Double labeled: blue - Tgfa; red - Dlx2 (assay MGI:7518938).
Specimen
3D:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled: blue - Tgfa; red - Dlx2 (assay MGI:7518938).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebral cortex |
Present |
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3D
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Expression in cortical neuroepithelium in wedge-shaped territory lacking Emx1 and Dlx2 expression.
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Specimen
3G:
embryonic day 12.5
(more )
Note: Double labeled: blue - Tgfa; red - Sfrp2 (assay MGI:7518929).
Specimen
3G:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled: blue - Tgfa; red - Sfrp2 (assay MGI:7518929).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cortical antihem |
Present |
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3G
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Expression marks most lateral margin of cortical neuroepithelium.
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Specimen
4A:
embryonic day 14.5
(more )
Specimen
4A:
(close )
Genetic Background:
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involves: C3H/He
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
4B:
(close )
Genetic Background:
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involves: C3H/He
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
4C:
embryonic day 13.5
(more )
Specimen
4C:
(close )
Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
4D:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Emx2tm1Pgr/Emx2tm1Pgr
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
|
whole mount
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Structure |
Level |
Pattern |
Image |
Note |
TS21: cortical antihem |
Present |
Regionally restricted |
4D
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Expression in antihem present in mutant at E13.5 but appears dorsally displaced.
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Specimen
4E:
embryonic day 14.5
(more )
Specimen
4E:
(close )
Genetic Background:
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involves: C3H/He
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Specimen
4F:
(close )
Genetic Background:
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involves: C3H/He
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Structure |
Level |
Pattern |
Image |
Note |
TS22: telencephalon |
Present |
Regionally restricted |
4F
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Expression eliminated in antihem region.
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