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Gene Expression Data
Assay Details
Assay
Reference: J:310864 Zainolabidin N, et al., Distinct Activities of Tfap2A and Tfap2B in the Specification of GABAergic Interneurons in the Developing Cerebellum. Front Mol Neurosci. 2017;10:281
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7520312
Gene symbol: Tfap2b
Gene name: transcription factor AP-2 beta
Antibody: anti-AP-2b
Detection system: Secondary antibody coupled to Alexa Fluor 555
Results
Specimen 3K/N: embryonic day 12.5 (more )
Note: Triple labeled: red - Tfap2b; blue - Lhx1 (assay MGI:7520314) and Lhx5 (assay MGI:7520410); green - Olig2 (assay MGI:7520313).
Structure Cell Type Level Pattern Image Note
TS20: cerebellum primordium cerebellar inhibitory GABAergic interneuron Present Regionally restricted 3K , 3N Some Tfap2b+ cells expressed Olig2 while others express both Olig2 and Lhx1/5 (both mitotic and early postmitotic GABAergic cells) in the ventricular zone. All Olig2-expressing cells in the ventricular zone expressed Tfap2b.
TS20: cerebellum white matter cerebellar inhibitory GABAergic interneuron Present 3N , 3K Only coexpressed Lhx1/5 (postmitotic GABAergic neurons).

Specimen 4D/E/F: embryonic day 15.5 (more )
Note: Triple labeled: red - Tfap2b; blue - Pax2 (assay MGI:7520316); green - Rora (assay MGI:7520315).
Structure Cell Type Level Pattern Image Note
TS23: cerebellum cerebellar inhibitory GABAergic interneuron Present 4D , 4E' , 4F' Expressed in two subpopulations (Tfap2b+/Pax2+ and Tfap2b+-alone).
TS23: cerebellum layer Purkinje cell Absent 4E' , 4D , 4F' Not coexpressed with Rora.

Specimen 4J/K/L: embryonic day 18.5 (more )
Note: Triple labeled: red - Tfap2b; blue - Pax2 (assay MGI:7520316); green - Rora (assay MGI:7520315).
Structure Cell Type Level Pattern Image Note
TS26: cerebellum white matter cerebellar inhibitory GABAergic interneuron Present 4L' , 4J , 4K' An increase in Tfap2b+/Pax2+ cells from 54% to 86% and a decrease in cells expressing only Tfap2b from 42% to 14%.
TS26: cerebellum Purkinje cell layer Purkinje cell Absent 4J , 4K' , 4L'

Specimen S3G/I: embryonic day 12.5 (more )
Note: Double labeled: red - Tfap2b; green - Pax2 (assay MGI:7520321).
Structure Level Pattern Image Note
TS20: cerebellum white matter Present S3G , S3I Coexpressed with Pax2 in GABAergic interneuron precursors.
TS20: cerebellum primordium Present Regionally restricted S3G , S3I Expressed in the ventricular zone. Coexpressed with Pax2 in GABAergic interneuron precursors.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory