Gene Expression Data
Assay Details
Assay
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Reference:
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J:65135
Schwarting GA, et al., Semaphorin 3A is required for guidance of olfactory axons in mice. J Neurosci. 2000 Oct 15;20(20):7691-7
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7522012
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Gene symbol:
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Sema3a
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Gene name:
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sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
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Probe:
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SemD cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A:
embryonic day 13.5
(more )
Note: Rostral olfactory bulb.
Specimen
1A:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 13.5
|
Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Rostral olfactory bulb.
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Specimen
1C:
embryonic day 13.5
(more )
Note: Medial olfactory bulb.
Specimen
1C:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 13.5
|
Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Medial olfactory bulb.
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Specimen
1E:
embryonic day 13.5
(more )
Note: Caudal olfactory bulb.
Specimen
1E:
(close )
Genetic Background:
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C57BL/6
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Age:
|
embryonic day 13.5
|
Sex:
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Not Specified
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Type:
|
section
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Embedding:
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Cryosection
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Note:
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Caudal olfactory bulb.
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Structure |
Level |
Pattern |
Image |
Note |
TS21: telencephalon |
Present |
Regionally restricted |
1E
,
1G
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Expression is in a sheet of cells in the nerve layer surrounding the olfactory bulb.
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TS21: main olfactory bulb layer |
Present |
Regionally restricted |
1E
,
1G
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In the caudal olfactory bulb expression in the ventral nerve layer has gaps in expression.
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Specimen
2A:
embryonic day 15.5
(more )
Specimen
2A:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: main olfactory bulb olfactory nerve layer |
Present |
Regionally restricted |
2A
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Expression is in the outer nerve layer in a block of cells distributed along the ventral surface of the olfactory bulb, with small gaps near the rostral and caudal ends.
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Specimen
2C:
embryonic day 15.5
(more )
Specimen
2C:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: main olfactory bulb olfactory nerve layer |
Present |
Regionally restricted |
2C
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There large gaps in expression in the medial and ventral nerve layer that flank a region of heavy expression in the ventromedial nerve layer.
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Specimen
not shown E12:
embryonic day 12.5
(more )
Specimen
not shown E12:
(close )
Genetic Background:
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C57BL/6
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Age:
|
embryonic day 12.5
|
Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS20: olfactory epithelium |
Present |
Regionally restricted |
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Expression is in non-neuronal cells along axon pathways between the olfactrory epithelium and the olfactory bulb.
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TS20: telencephalon |
Present |
Regionally restricted |
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Expression is in non-neuronal cells along axon pathways between the olfactrory epithelium and the olfactory bulb.
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