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Gene Expression Data
Assay Details
Assay
Reference: J:183829 Correa SM, et al., Sex reversal in C57BL/6J XY mice caused by increased expression of ovarian genes and insufficient activation of the testis determining pathway. PLoS Genet. 2012 Apr;8(4):e1002569
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7524458
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: anti-CD31
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 1M Wt1+/+ XX: embryonic day 13.5 (more )
Note: Double labeled: red - Pecam1; green - Nr5a1 (assay MGI:7524459).
Structure Cell Type Level Pattern Image Note
TS21: developing vasculature of ovary blood vessel endothelial cell Present 1M
TS21: germ cell of ovary Present 1M
Specimen 1N Wt1+/+ XY AKR: embryonic day 13.5 (more )
Note: Double labeled: red - Pecam1; green - Nr5a1 (assay MGI:7524459).
Structure Cell Type Level Pattern Image Note
TS21: developing vasculature of reproductive system blood vessel endothelial cell Present 1N
TS21: germ cell of gonad Present 1N
Specimen 1O Wt1+/- XY AKR: embryonic day 13.5; Wt1tm1Jae/Wt1+ (more )
Note: Gonads differentiated as ovaries. Double labeled: red - Pecam1; green - Nr5a1 (assay MGI:7524459).
Structure Cell Type Level Pattern Image Note
TS21: developing vasculature of reproductive system blood vessel endothelial cell Present 1O
TS21: germ cell of gonad Present 1O
Specimen 3C/D Wt1+/+;Sf1+/+ XY B6: embryonic day 13.5 (more )
Note: Double labeled: red - Pecam1; green - Sox9 (assay MGI:7524635).
Structure Cell Type Level Pattern Image Note
TS21: developing vasculature of testis blood vessel endothelial cell Present 3D , 3C
TS21: germ cell of testis Present 3D , 3C
Specimen 3E/F Wt1+/-;Sf1+/+ XY B6: embryonic day 13.5; Wt1tm1Jae/Wt1+ (more )
Note: Generated from B6 Wt1+/- and B6 +/+ XY B6 crosses. Double labeled: red - Pecam1; green - Sox9 (assay MGI:7524635).
Structure Cell Type Level Pattern Image Note
TS21: developing vasculature of testis blood vessel endothelial cell Present 3F , 3E
TS21: germ cell of testis Present 3F , 3E
Specimen 3G/H Wt1+/+;Sf1+/- XY B6: embryonic day 13.5; Nr5a1tm1Klp/Nr5a1+ (more )
Note: Double labeled: red - Pecam1; green - Sox9 (assay MGI:7524635).
Structure Cell Type Level Pattern Image Note
TS21: developing vasculature of testis blood vessel endothelial cell Present 3G , 3H
TS21: germ cell of testis Present 3G , 3H
Specimen 3I/J Wt1+/-;Sf1+/- XY B6: embryonic day 13.5; Nr5a1tm1Klp/Nr5a1+, Wt1tm1Jae/Wt1+ (more )
Note: Gonads differentiated as ovotestes. Double labeled: red - Pecam1; green - Sox9 (assay MGI:7524635).
Structure Cell Type Level Pattern Image Note
TS21: developing vasculature of reproductive system blood vessel endothelial cell Present 3I , 3J
TS21: germ cell of gonad Present 3J , 3I
Specimen 4BA +/+ XY AKR: embryonic day 13.5 (more )
Note: Double labeled: red - Pecam1; green - Amh (assay MGI:7524638).
Structure Cell Type Level Pattern Image Note
TS21: developing vasculature of reproductive system blood vessel endothelial cell Present 4BA
TS21: germ cell of gonad Present 4BA
Specimen 4BB Wt1+/- XY AKR: embryonic day 13.5; Wt1tm1Jae/Wt1+ (more )
Note: Double labeled: red - Pecam1; green - Amh (assay MGI:7524638).
Structure Cell Type Level Pattern Image Note
TS21: developing vasculature of testis blood vessel endothelial cell Present 4BB
TS21: germ cell of testis Present 4BB
Specimen 4BC Sf1+/- XY AKR: embryonic day 13.5; Nr5a1tm1Klp/Nr5a1+ (more )
Note: Double labeled: red - Pecam1; green - Amh (assay MGI:7524638).
Structure Cell Type Level Pattern Image Note
TS21: developing vasculature of reproductive system blood vessel endothelial cell Present 4BC
TS21: germ cell of gonad Present 4BC
Specimen 4BD/G +/+ XY AKR,Sxr: embryonic day 13.5; X/SryAKR/JSryRIII (more )
Note: Double labeled: red - Pecam1; green - Amh (assay MGI:7524638).
Structure Cell Type Level Pattern Image Note
TS21: developing vasculature of testis blood vessel endothelial cell Present 4BD , 4BG
TS21: germ cell of testis Present 4BD , 4BG
Specimen 4BE/H Wt1+/- XY AKR,Sxr: embryonic day 13.5; X/SryAKR/JSryRIII, Wt1tm1Jae/Wt1+ (more )
Note: Double labeled: red - Pecam1; green - Amh (assay MGI:7524638).
Structure Cell Type Level Pattern Image Note
TS21: developing vasculature of testis blood vessel endothelial cell Present 4BE , 4BH
TS21: germ cell of testis Present 4BE , 4BH
Specimen 4BF/I Sf1+/- XY AKR,Sxr: embryonic day 13.5; Nr5a1tm1Klp/Nr5a1+, X/SryAKR/JSryRIII (more )
Note: Double labeled: red - Pecam1; green - Amh (assay MGI:7524638).
Structure Cell Type Level Pattern Image Note
TS21: developing vasculature of testis blood vessel endothelial cell Present 4BI , 4BF
TS21: germ cell of testis Present 4BF , 4BI
Specimen 6A Wt1+/-;Wnt4+/+ XY AKR: embryonic day 13.5; Wt1tm1Jae/Wt1+ (more )
Note: Double labeled: red - Pecam1; green - Amh (assay MGI:7524638).
Structure Cell Type Level Pattern Image Note
TS21: developing vasculature of reproductive system blood vessel endothelial cell Present 6A
TS21: germ cell of gonad Present 6A
Specimen 6B Wt1+/-;Wnt4+/- XY AKR: embryonic day 13.5; Wnt4tm1Amc/Wnt4+, Wt1tm1Jae/Wt1+ (more )
Note: Double labeled: red - Pecam1; green - Amh (assay MGI:7524638).
Structure Cell Type Level Pattern Image Note
TS21: developing vasculature of reproductive system blood vessel endothelial cell Present 6B
TS21: germ cell of gonad Present 6B
Specimen 6C Sf1+/-;Wnt4+/+ XY AKR: embryonic day 13.5; Nr5a1tm1Klp/Nr5a1+ (more )
Note: Gonads differentiated as ovaries. Double labeled: red - Pecam1; green - Amh (assay MGI:7524638).
Structure Cell Type Level Pattern Image Note
TS21: developing vasculature of reproductive system blood vessel endothelial cell Present 6C
TS21: germ cell of gonad Present 6C
Specimen 6D Sf1+/-;Wnt4+/- XY AKR: embryonic day 13.5; Nr5a1tm1Klp/Nr5a1+, Wnt4tm1Amc/Wnt4+ (more )
Note: Double labeled: red - Pecam1; green - Amh (assay MGI:7524638).
Structure Cell Type Level Pattern Image Note
TS21: developing vasculature of reproductive system blood vessel endothelial cell Present 6D
TS21: germ cell of gonad Present 6D
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
MGI 6.13 		The Jackson Laboratory