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Gene Expression Data
Assay Details
Assay
Reference: J:183829 Correa SM, et al., Sex reversal in C57BL/6J XY mice caused by increased expression of ovarian genes and insufficient activation of the testis determining pathway. PLoS Genet. 2012 Apr;8(4):e1002569
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7524638
Gene symbol: Amh
Gene name: anti-Mullerian hormone
Antibody: anti-AMH
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 4BA +/+ XY AKR: embryonic day 13.5 (more )
Note: Double labeled: green - Amh; red - Pecam1 (assay MGI:7524458).
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present 4BA
Specimen 4BB Wt1+/- XY AKR: embryonic day 13.5; Wt1tm1Jae/Wt1+ (more )
Note: Double labeled: green - Amh; red - Pecam1 (assay MGI:7524458).
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present Regionally restricted 4BB Expressed in the central region containing testicular cords but not at poles that lacked cords. Testis cords were less developed and more disorganized than cords in the wild-type.
Specimen 4BC Sf1+/- XY AKR: embryonic day 13.5; Nr5a1tm1Klp/Nr5a1+ (more )
Note: Double labeled: green - Amh; red - Pecam1 (assay MGI:7524458).
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present Regionally restricted 4BC Testis cords were less developed (only in the center) and more disorganized than cords in the wild-type.
Specimen 4BD/G +/+ XY AKR,Sxr: embryonic day 13.5; X/SryAKR/JSryRIII (more )
Note: Double labeled: green - Amh; red - Pecam1 (assay MGI:7524458).
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present 4BD , 4BG Similar as in the wild-type.
Specimen 4BE/H Wt1+/- XY AKR,Sxr: embryonic day 13.5; X/SryAKR/JSryRIII, Wt1tm1Jae/Wt1+ (more )
Note: Double labeled: green - Amh; red - Pecam1 (assay MGI:7524458).
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present 4BH , 4BE Developed more testicular tissue than Wt1+/- B6 XY AKR gonads, but still less testicular tissue, less differentiated and less organized than +/+ B6 XY AKR-Sxr controls.
Specimen 4BF/I Sf1+/- XY AKR,Sxr: embryonic day 13.5; Nr5a1tm1Klp/Nr5a1+, X/SryAKR/JSryRIII (more )
Note: Double labeled: green - Amh; red - Pecam1 (assay MGI:7524458).
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present 4BF , 4BI Developed more testicular tissue than Sf1+/- B6 XY AKR gonads, but still less testicular tissue, less differentiated and less organized than +/+ B6 XY AKR-Sxr controls.
Specimen 6A Wt1+/-;Wnt4+/+ XY AKR: embryonic day 13.5; Wt1tm1Jae/Wt1+ (more )
Note: Double labeled: green - Amh; red - Pecam1 (assay MGI:7524458).
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present Regionally restricted 6A Expressed in the central region containing testicular cords but not at poles that lacked cords.
Specimen 6B Wt1+/-;Wnt4+/- XY AKR: embryonic day 13.5; Wnt4tm1Amc/Wnt4+, Wt1tm1Jae/Wt1+ (more )
Note: Double labeled: green - Amh; red - Pecam1 (assay MGI:7524458).
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present 6B The amount of positive tissue was consistently greater than the Wt1+/-;Wnt4+/+ XY AKR controls.
Specimen 6C Sf1+/-;Wnt4+/+ XY AKR: embryonic day 13.5; Nr5a1tm1Klp/Nr5a1+ (more )
Note: Gonads differentiated as ovaries. Double labeled: green - Amh; red - Pecam1 (assay MGI:7524458).
Structure Level Pattern Image Note
TS21: gonad Absent 6C Developed as ovaries, similar to XX ovaries.
Specimen 6D Sf1+/-;Wnt4+/- XY AKR: embryonic day 13.5; Nr5a1tm1Klp/Nr5a1+, Wnt4tm1Amc/Wnt4+ (more )
Note: Double labeled: green - Amh; red - Pecam1 (assay MGI:7524458).
Structure Cell Type Level Pattern Image Note
TS21: Sertoli cell Sertoli cell Present 6D The amount of positive tissue was consistently greater than the Sf1+/-;Wnt4+/+ XY AKR controls.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/24/2024
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