Probe:	Dkk3 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS23: cochlea	Present	Regionally restricted	5D , 5C , 5B , 5A	Expression was detected in the greater epithelial ridge in the mid turn, but was not detected in the most immature apical turn.
TS23: greater epithelial ridge	Strong	Regionally restricted	5C , 5D , 5A	Expression initiated in the greater epithelial ridge in the midturn, with strongest expression in the greater epithelial ridge adjacent to inner hair cells in the basal turn.

Structure	Level	Pattern	Image	Note
TS27: cochlea	Present	Regionally restricted	5H , 5E , 5F , 5G	Expression was maintained in all three turns of the cochlea.
TS27: greater epithelial ridge	Strong	Non-Uniform	5E , 5G , 5H , 5F	In the least mature, apical, turn expression was detected in the greater epithelial ridge bordering the sensory epithelium. In the more advanced mid turn expression remained and was more intensely stained than in the apex. In the most mature base turn, the expression was at its strongest.

Structure	Level	Pattern	Image	Note
TS28: cochlea	Absent		5I
TS28: greater epithelial ridge	Absent		5I

Structure	Level	Pattern	Image	Note
TS28: stria vascularis	Present		5J
TS28: spiral limbus	Present	Regionally restricted	5J	Expression was detected at the inner edge of the spiral limbus.