Gene Expression Data
Assay Details
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Assay
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Reference:
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J:268103
Dykes IM, et al., A Requirement for Zic2 in the Regulation of Nodal Expression Underlies the Establishment of Left-Sided Identity. Sci Rep. 2018 Jul 11;8(1):10439
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7530983
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Gene symbol:
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Pitx2
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Gene name:
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paired-like homeodomain transcription factor 2
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Probe:
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Pitx2 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
4a:
embryonic day 8.0
(more  )
Note: 4-somite stage
Specimen
4a:
(close  )
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Genetic Background:
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involves: C3H/HeH * C57BL/6
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-somite stage
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Specimen
4b:
embryonic day 8.0; Zic2iso/ Zic2iso
(more )
Note: 4-somite stage
Specimen
4b:
(close )
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Genetic Background:
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involves: C3H/HeH * C57BL/6
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Mutant Allele(s):
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Zic2iso/Zic2iso
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-somite stage
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Specimen
not shown WT/Het:
embryonic day 8.0
(more )
Note: 4-7 somites, summarized in Fig 4c
Specimen
not shown WT/Het:
(close )
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Genetic Background:
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involves: C3H/HeH * C57BL/6
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-7 somites, summarized in Fig 4c
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Specimen
not shown iso/iso:
embryonic day 8.0; Zic2iso/ Zic2iso
(more )
Note: 6-7 somites, summarized in Fig 4c
Specimen
not shown iso/iso:
(close )
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Genetic Background:
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involves: C3H/HeH * C57BL/6
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Mutant Allele(s):
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Zic2iso/Zic2iso
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6-7 somites, summarized in Fig 4c
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Weak |
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Expression appeared to be delayed from the 4 to the 6-somite stage. Expression was weaker, and its laterality was perturbed often bilateral (3 out of 9 embryos). Weak expression on the left in one 6-somite embryo.
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Specimen
not shown iso/iso:
embryonic day 8.0; Zic2iso/ Zic2iso
(more )
Note: 5-6 somites, summarized in Fig 4c
Specimen
not shown iso/iso:
(close )
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Genetic Background:
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involves: C3H/HeH * C57BL/6
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Mutant Allele(s):
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Zic2iso/Zic2iso
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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5-6 somites, summarized in Fig 4c
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Analysis Tools