Gene Expression Data
Assay Details
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Assay
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Reference:
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J:268103
Dykes IM, et al., A Requirement for Zic2 in the Regulation of Nodal Expression Underlies the Establishment of Left-Sided Identity. Sci Rep. 2018 Jul 11;8(1):10439
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7530987
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
4t:
embryonic day 8.0
(more  )
Specimen
4t:
(close  )
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Genetic Background:
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involves: C3H/HeH * C57BL/6
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural tube |
Present |
Regionally restricted |
4t
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Expressed along the embryonic midline in a solid band.
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Specimen
4u:
(close )
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Genetic Background:
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involves: C3H/HeH * C57BL/6
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Mutant Allele(s):
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Zic2iso/Zic2iso
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural tube |
Present |
Regionally restricted |
4u
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Expression levels were maintained but there seemed to be fewer positive cells and the band of expression appeared disrupted.
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Analysis Tools