About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:293794 Durbin MD, et al., SHROOM3 is downstream of the planar cell polarity pathway and loss-of-function results in congenital heart defects. Dev Biol. 2020 Aug 15;464(2):124-136
Assay type: Western blot
MGI Accession ID: MGI:7531400
Gene symbol: Dvl2
Gene name: dishevelled segment polarity protein 2
Antibody: anti-Dishevelled 2 (phospho S143) (ab124933)
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: The antibody used in this assay recognizes the activated form of the protein.
Results
Image: 5X pDVL2 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Genetic BackgroundMutant Allele(s)Sex Note
Shroom3+/+ E12.5,14.5 TS20: heart
TS22: heart 		Present C57BL/6J Not Specified It was unclear whether the pooled hearts were of both ages or if each age was examined individually.
Shroom3+/+ E12.5,14.5 TS20: heart
TS22: heart 		Present C57BL/6J Not Specified It was unclear whether the pooled hearts were of both ages or if each age was examined individually.
Shroom3+/+ E12.5,14.5 TS20: heart
TS22: heart 		Present C57BL/6J Not Specified It was unclear whether the pooled hearts were of both ages or if each age was examined individually.
Shroom3gt/gt E12.5,14.5 TS20: heart
TS22: heart 		Present (a) B6.129S4-Shroom3Gt(ROSA53)Sor/J Shroom3Gt(ROSA53)Sor/Shroom3Gt(ROSA53)SorNot Specified It was unclear whether the pooled hearts were of both ages or if each age was examined individually.
Shroom3gt/gt E12.5,14.5 TS20: heart
TS22: heart 		Present (a) B6.129S4-Shroom3Gt(ROSA53)Sor/J Shroom3Gt(ROSA53)Sor/Shroom3Gt(ROSA53)SorNot Specified It was unclear whether the pooled hearts were of both ages or if each age was examined individually.
Shroom3gt/gt E12.5,14.5 TS20: heart
TS22: heart 		Present (a) B6.129S4-Shroom3Gt(ROSA53)Sor/J Shroom3Gt(ROSA53)Sor/Shroom3Gt(ROSA53)SorNot Specified It was unclear whether the pooled hearts were of both ages or if each age was examined individually.
Notes:
(a) Expression levels are reduced in the mutant relative to those seen in wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory