Sp7 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:329220 Chen Y, et al., Hnrnpk maintains chondrocytes survival and function during growth plate development via regulating Hif1alpha-glycolysis axis. Cell Death Dis. 2022 Sep 20;13(9):803
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7546918
Gene symbol:	Sp7
Gene name:	Sp7 transcription factor 7

Antibody:	Anti-Sp7/Osterix antibody [EPR21034] (ab209484)
Detection system:	Secondary antibody

Results

S1A Sp7 ©

Specimen S1A Sp7: embryonic day 13.5; Hnrnpk^tm1.1Cya/Hnrnpk^tm1.1Cya (more

)

Structure	Level	Pattern	Image	Note
TS21: humerus cartilage condensation	Present		S1A Sp7

S1B Sp7 ©

Specimen S1B Sp7: embryonic day 15.5; Hnrnpk^tm1.1Cya/Hnrnpk^tm1.1Cya (more

)

Structure	Level	Pattern	Image	Note
TS23: humerus	Present		S1B Sp7

S1C Sp7 ©

Specimen S1C Sp7: embryonic day 18.5; Hnrnpk^tm1.1Cya/Hnrnpk^tm1.1Cya (more

)

Structure	Level	Pattern	Image	Note
TS26: humerus	Present		S1C Sp7

2K WT ©

Specimen 2K WT: embryonic day 18.5; Hnrnpk^tm1.1Cya/Hnrnpk^tm1.1Cya (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS26: tibia	osteoblast	Present		2K WT	Expression in early osteoblasts.

2K CKO ©

Specimen 2K CKO: embryonic day 18.5; Hnrnpk^tm1.1Cya/Hnrnpk^tm1.1Cya, Tg(Col2a1-cre)1Xya/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS26: tibia	Strong	Regionally restricted	2K CKO	Expression markedly increased in bone marrow and perichondrium.

3D Sp7 WT ©

Specimen 3D Sp7 WT: postnatal day 0; Hnrnpk^tm1.1Cya/Hnrnpk^tm1.1Cya (more

)

Structure	Level	Pattern	Image	Note
TS27: femur	Present		3D Sp7 WT

3D Sp7 CKO ©

Specimen 3D Sp7 cCKO: postnatal day 0; Hnrnpk^tm1.1Cya/Hnrnpk^tm1.1Cya, Tg(Col10a1-cre)#Vdm/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: long bone metaphysis		Strong		3D Sp7 CKO	Increased expression in metaphysis.
TS27: femur	osteoblast	Present		3D Sp7 CKO	No change in number of osteoblasts compared to wild type.

Specimen 3E (upper): postnatal day 0; Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺, Tg(Col10a1-cre)#Vdm/0 (more

)
Note: Conditional mutant. Double labeled: green - Sp7; red - recombinase reporter.

Structure	Level	Pattern	Image	Note
TS27: femur	Present		3E (upper middle) , 3E (upper left) , 3E (upper right)

Specimen 3E (lower): postnatal day 0; Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺, Hnrnpk^tm1.1Cya/Hnrnpk^tm1.1Cya, Tg(Col10a1-cre)#Vdm/0 (more

)
Note: Conditional mutant. Double labeled: green - Sp7; red - recombinase reporter.

Structure	Level	Pattern	Image	Note
TS27: femur	Present		3E (lower right) , 3E (lower left) , 3E (lower middle)	Increased number of expressing cells compared to conrol.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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