Gene Expression Data
Assay Details
Assay
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Reference:
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J:217461
Yang G, et al., An eIF4E1/4E-T complex determines the genesis of neurons from precursors by translationally repressing a proneurogenic transcription program. Neuron. 2014 Nov 19;84(4):723-39
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7564091
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Gene symbol:
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Neurog2
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Gene name:
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neurogenin 2
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Results
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Specimen
7A neurogenin2:
embryonic day 15.0
(more )
Specimen
7A neurogenin2:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
S5A neurogenin2:
embryonic day 12.5
(more )
Specimen
S5A neurogenin2:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
7C neurogenin2:
embryonic day 15.0
(more )
Note: Double labeled: red - Neurog2; green - Eif4enif1 (assay MGI:7564070).
Specimen
7C neurogenin2:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Neurog2; green - Eif4enif1 (assay MGI:7564070).
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Structure |
Level |
Pattern |
Image |
Note |
TS23: cerebral cortex ventricular layer |
Present |
Regionally restricted |
7C neurogenin2 YZ
,
7C Merge XZ
,
7C neurogenin2
,
7C Merge
,
7C Merge YZ
,
7C neurogenin2 XZ
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Expression partially colocalized with Eif4nif1 expressing foci.
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Specimen
8A neurogenin2:
embryonic day 12.0
(more )
Note: Double labeled: red - Neurog2; green - Eif4enif1 (assay MGI:7564070).
Specimen
8A neurogenin2:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
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Double labeled: red - Neurog2; green - Eif4enif1 (assay MGI:7564070).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebral cortex subventricular zone |
Present |
Regionally restricted |
8A (left)
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Expression predominantly in cycling precursors. Approximately 37.7% of foci colocalized with Eif4nif1 at this stage.
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TS20: cerebral cortex ventricular layer |
Present |
Regionally restricted |
8A (left)
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Expression predominantly in cycling precursors. Approximately 37.7% of foci colocalized with Eif4nif1 at this stage.
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Specimen
8A neurogenin2 (top):
embryonic day 12.0
(more )
Note: Double labeled: red - Neurog2; green - Eif4enif1 (assay MGI:7564070).
Specimen
8A neurogenin2 (top):
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Neurog2; green - Eif4enif1 (assay MGI:7564070).
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Specimen
8A neurogenin2 (middle):
embryonic day 12.0
(more )
Note: Double labeled: red - Neurog2; green - Eif4enif1 (assay MGI:7564070).
Specimen
8A neurogenin2 (middle):
(close )
Genetic Background:
|
CD-1
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Age:
|
embryonic day 12.0
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Neurog2; green - Eif4enif1 (assay MGI:7564070).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebral cortex subventricular zone |
Present |
Regionally restricted |
8A (middle)
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Highest proportion of expressing foci at interface of ventricular zone and subventricular zones (bin 2).
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TS20: cerebral cortex ventricular layer |
Present |
Regionally restricted |
8A (middle)
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Highest proportion of expressing foci at interface of ventricular zone and subventricular zones (bin 2).
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Specimen
8A neurogenin2 (bottom):
embryonic day 12.0
(more )
Note: Double labeled: red - Neurog2; green - Eif4enif1 (assay MGI:7564070).
Specimen
8A neurogenin2 (bottom):
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
|
Note:
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Double labeled: red - Neurog2; green - Eif4enif1 (assay MGI:7564070).
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Specimen
8E neurogenin2 mRNA:
embryonic day 12.0
(more )
Note: Triple labeled: red - Neurog2 RNA; green - Eif4enif1 (assay MGI:7564070); blue - Neurog2 protein (assay MGI:7564072).
Specimen
8E neurogenin2 mRNA:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Note:
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Triple labeled: red - Neurog2 RNA; green - Eif4enif1 (assay MGI:7564070); blue - Neurog2 protein (assay MGI:7564072).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebral cortex |
Present |
Regionally restricted |
8E (left)
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Expression in all Neurog2 protein expressing cells, but also in cells not expressing Neurog2 protein.
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Specimen
8E neurogenin2 mRNA (upper):
embryonic day 12.0
(more )
Note: Triple labeled: red - Neurog2 RNA; green - Eif4enif1 (assay MGI:7564070); blue - Neurog2 protein (assay MGI:7564072).
Specimen
8E neurogenin2 mRNA (upper):
(close )
Genetic Background:
|
CD-1
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Age:
|
embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Neurog2 RNA; green - Eif4enif1 (assay MGI:7564070); blue - Neurog2 protein (assay MGI:7564072).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebral cortex |
Present |
Regionally restricted |
8E neurogenin2 mRNA (upper)
,
8E Merge (upper)
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Expression in all Neurog2 protein expressing cells, but also in cells not expressing Neurog2 protein.
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Specimen
8E neurogenin2 mRNA (lower):
embryonic day 12.0
(more )
Note: Triple labeled: red - Neurog2 RNA; green - Eif4enif1 (assay MGI:7564070); blue - Neurog2 protein (assay MGI:7564072).
Specimen
8E neurogenin2 mRNA (lower):
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.0
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - Neurog2 RNA; green - Eif4enif1 (assay MGI:7564070); blue - Neurog2 protein (assay MGI:7564072).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebral cortex |
Present |
Regionally restricted |
8E neurogenin2 mRNA (lower)
,
8E Merge (lower)
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Expression in all Neurog2 protein expressing cells, but also in cells not expressing Neurog2 protein.
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