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Gene Expression Data
Assay Details
Assay
Reference: J:195751 Mamber C, et al., GFAPdelta expression in glia of the developmental and adolescent mouse brain. PLoS One. 2012;7(12):e52659
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7564571
Gene symbol: Gfap
Gene name: glial fibrillary acidic protein
Antibody: anti-GFAP-delta
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 1A: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: brain Absent 1A
Specimen 1C: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: brain Absent 1C
Specimen 1E: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: allocortex Present 1E
TS26: brain ventricular layer Present 1E
TS26: hindbrain Present Regionally restricted 1E Expression is along the pial surface.
Specimen 1G: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: allocortex Present 1G
TS27: brain ventricular layer Present 1G
TS27: hindbrain Present Regionally restricted 1G Expression is along the pial surface.
Specimen 2A: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: telencephalon Absent 2A
Specimen 2B: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: telencephalon ventricular layer Present Single cells 2B Expression is in a population of bipolar cells that extend their processes towards the lateral ventricle and stretch towards the pia.
Specimen 2C: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: forebrain ventricular layer Present Single cells 2C Expression is in a population of bipolar cells that extend their processes towards the lateral ventricle and stretch towards the pia.
Specimen 2G: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: allocortex Absent 2G Absent in the dentate gyrus.
Specimen 2H: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: dentate gyrus Strong Regionally restricted 2H Expression is in a cluster of bushy cells with thick, stubby processes.
Specimen 2I: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: dentate gyrus Strong Regionally restricted 2I Expression is in a cluster of bushy cells with thick, stubby processes.
Specimen 3A: embryonic day 18.5 (more )
Note: Triple labeled: red - Gfap; white - Mcm2 (assay MGI:7564581); green - Vim (assay MGI:7564579).
Structure Level Pattern Image Note
TS26: forebrain ventricular layer Present 3A , 3A GFAPdelta Expression always colocalized with vimentin.
Specimen 3B: embryonic day 18.5 (more )
Note: Triple labeled: red - Gfap protein; green - Gfap protein (assay MGI:7564582); white - Nes (assay MGI:7564580).
Structure Level Pattern Image Note
TS26: forebrain ventricular layer Present 3B GFAPdelta , 3B There was colocalizion with Gfap-C-terminus and alwasy with Nes.
Specimen 3C: postnatal day 0 (more )
Note: Triple labeled: red - Gfap; white - Mcm2 (assay MGI:7564581); green - Vim (assay MGI:7564579).
Structure Level Pattern Image Note
TS27: forebrain ventricular layer Present 3C GFAPdelta , 3C Expression always colocalized with vimentin.
TS27: telencephalon subventricular zone Present 3C GFAPdelta , 3C Expression always colocalized with vimentin.
Specimen 3D: postnatal day 0 (more )
Note: Triple labeled: red - Gfap protein; green - Gfap protein (assay MGI:7564582); white - Nes (assay MGI:7564580).
Structure Level Pattern Image Note
TS27: forebrain ventricular layer Present 3D , 3D GFAPdelta
TS27: telencephalon subventricular zone Present 3D , 3D GFAPdelta
Specimen 4A: embryonic day 18.5 (more )
Note: Triple labeled: red - Gfap; white - Mcm2 (assay MGI:7564581); green - Vim (assay MGI:7564579).
Structure Level Pattern Image Note
TS26: dentate gyrus Present 4A , 4A GFAPdelta
Specimen 4B: embryonic day 18.5 (more )
Note: Triple labeled: red - Gfap protein; green - Gfap protein (assay MGI:7564582); white - Nes (assay MGI:7564580).
Structure Level Pattern Image Note
TS26: allocortex Present 4B GFAPdelta , 4B
Specimen 4C: postnatal day 0 (more )
Note: Triple labeled: red - Gfap; white - Mcm2 (assay MGI:7564581); green - Vim (assay MGI:7564579).
Structure Level Pattern Image Note
TS27: fimbria hippocampus Present 4C GFAPdelta , 4C
Specimen 4D: postnatal day 0 (more )
Note: Triple labeled: red - Gfap protein; green - Gfap protein (assay MGI:7564582); white - Nes (assay MGI:7564580).
Structure Level Pattern Image Note
TS27: dentate gyrus Present 4D GFAPdelta , 4D
Specimen 6A: postnatal day 5 (more )
Note: Double labeled: red - Gfap protein; green - Gfap protein (assay MGI:7564570).
Structure Level Pattern Image Note
TS28: dentate gyrus Present Regionally restricted 6C , 6A Expression is in cells that have a relatively bushy morphology and are clustered closely together. There is complete overlap in staining with the other antibody.
Specimen 6D: postnatal day 10 (more )
Note: Double labeled: red - Gfap protein; green - Gfap protein (assay MGI:7564570).
Structure Level Pattern Image Note
TS28: dentate gyrus Present Regionally restricted 6D , 6F Expression is in cells with a bipolar morphology. There is complete overlap in staining with the other antibody.
Specimen 6G: postnatal day 25 (more )
Note: Double labeled: red - Gfap protein; green - Gfap protein (assay MGI:7564570).
Structure Level Pattern Image Note
TS28: dentate gyrus Present Regionally restricted 6I , 6G Expression is in long-range fibers. There is complete overlap in staining with the other antibody.
Specimen 7A: postnatal day 5 (more )
Note: Double labeled: red - Gfap protein; green - Gfap protein (assay MGI:7564570).
Structure Level Pattern Image Note
TS28: brain subventricular zone Present Single cells 7C , 7A Expression is in cells that resemble bipolar radial glia.
Specimen 7D: postnatal day 10 (more )
Note: Double labeled: red - Gfap protein; green - Gfap protein (assay MGI:7564570).
Structure Level Pattern Image Note
TS28: brain subventricular zone Present Regionally restricted 7D , 7F Expression is in thick fibers transversing the subventricular zone.
Specimen 7G: postnatal day 25 (more )
Note: Double labeled: red - Gfap protein; green - Gfap protein (assay MGI:7564570).
Structure Level Pattern Image Note
TS28: brain subventricular zone Present Regionally restricted 7I , 7G Expression is punctate and in thick fibers transversing the subventricular zone.
Specimen 8A: postnatal day 10 (more )
Note: Triple labeled: red - Gfap; white - Mcm2 (assay MGI:7564581); green - Vim (assay MGI:7564579).
Structure Level Pattern Image Note
TS28: telencephalon subventricular zone Present 8B , 8B GFAPdelta , 8A
Specimen 8C: postnatal day 10 (more )
Note: Double labeled: red - Gfap; green - phosphohistone-H3.
Structure Level Pattern Image Note
TS28: telencephalon subventricular zone Present 8C There was no co-localization.
Specimen 8D: postnatal day 10 (more )
Note: Triple labeled: red - Gfap protein; green - Gfap protein (assay MGI:7564582); white - Nes (assay MGI:7564580).
Structure Level Pattern Image Note
TS28: telencephalon subventricular zone Present 8D GFAPdelta , 8D
Specimen 8E: postnatal day 10 (more )
Note: Triple labeled: red - Gfap; white - Mcm2 (assay MGI:7564581); green - Vim (assay MGI:7564579).
Structure Level Pattern Image Note
TS28: hippocampus Present 8F , 8E , 8F GFAPdelta
Specimen 8G: postnatal day 10 (more )
Note: Triple labeled: - Gfap protein; green - Gfap protein (assay MGI:7564582); white - Nes (assay MGI:7564580).
Structure Level Pattern Image Note
TS28: dentate gyrus Present 8H , 8H GFAPdelta , 8G
Specimen S1A: embryonic day 18.5 (more )
Note: Triple labeled: red - Gfap protein; green - Gfap protein (assay MGI:7564582); white - Nes (assay MGI:7564580).
Structure Level Pattern Image Note
TS26: telencephalon ventricular layer Present S1A
Specimen S1B: embryonic day 18.5 (more )
Note: Triple labeled: red - Gfap protein; green - Gfap protein (assay MGI:7564582); white - Nes (assay MGI:7564580).
Structure Level Pattern Image Note
TS26: allocortex Present S1B
Specimen S1C: postnatal day 0 (more )
Note: Triple labeled: red - Gfap protein; green - Gfap protein (assay MGI:7564582); white - Nes (assay MGI:7564580).
Structure Level Pattern Image Note
TS27: forebrain ventricular layer Present S1C
Specimen S1D: postnatal day 0 (more )
Note: Triple labeled: red - Gfap protein; green - Gfap protein (assay MGI:7564582); white - Nes (assay MGI:7564580).
Structure Level Pattern Image Note
TS27: allocortex Present S1D
Specimen Table 2 E18: embryonic day 18.5 (more )
Structure Cell Type Level Pattern Image Note
TS26: hippocampus Strong Regionally restricted Expression is strong in fimbria and weak in the hippocampus.
TS26: brain subventricular zone Moderate
TS26: optic nerve Moderate
TS26: brain Weak Regionally restricted Expression is weak in the glia limitans.
TS26: cerebellum Absent
TS26: cerebral cortex Absent
TS26: corpus striatum Absent
TS26: main olfactory bulb Absent
TS26: retina Mueller cell Absent Expression is absent in Muller glia.
Specimen Table 2 P0: postnatal day 0 (more )
Structure Cell Type Level Pattern Image Note
TS27: fimbria hippocampus Strong
TS27: optic nerve Strong
TS27: brain subventricular zone Moderate
TS27: brain Moderate Regionally restricted Expression is moderate in the glia limitans.
TS27: cerebral cortex Weak
TS27: hippocampus Weak
TS27: cerebellum Absent
TS27: corpus striatum Absent
TS27: main olfactory bulb Absent
TS27: retina Mueller cell Absent Expression is absent in Muller glia.
Specimen Table 2 P5: postnatal day 5 (more )
Structure Cell Type Level Pattern Image Note
TS28: fimbria hippocampus Strong
TS28: optic nerve Strong
TS28: brain subventricular zone Moderate
TS28: brain Moderate Regionally restricted Expression is moderate in the glia limitans.
TS28: cerebellum Weak
TS28: cerebral cortex Weak
TS28: corpus striatum Weak
TS28: hippocampus Weak
TS28: main olfactory bulb Weak
TS28: retina Mueller cell Weak Regionally restricted Expression is weak in Muller glia.
Specimen Table 2 P10: postnatal day 10 (more )
Structure Level Pattern Image Note
TS28: brain subventricular zone Moderate
TS28: cerebellum Moderate
TS28: fimbria hippocampus Moderate
TS28: brain Moderate Regionally restricted Expression is moderate in the glia limitans.
TS28: cerebral cortex Weak
TS28: corpus striatum Weak
TS28: hippocampus Weak
TS28: main olfactory bulb Weak
Specimen Table 2 P25: postnatal day 25 (more )
Structure Level Pattern Image Note
TS28: brain subventricular zone Moderate
TS28: cerebellum Moderate
TS28: brain Moderate Regionally restricted Expression is moderate in the glia limitans.
TS28: cerebral cortex Weak
TS28: corpus striatum Weak
TS28: fimbria hippocampus Weak
TS28: hippocampus Weak
TS28: main olfactory bulb Weak
Specimen Table 2 P45: postnatal day 45 (more )
Structure Level Pattern Image Note
TS28: brain subventricular zone Moderate
TS28: cerebellum Moderate
TS28: brain Moderate Regionally restricted Expression is moderate in the glia limitans.
TS28: cerebral cortex Weak
TS28: corpus striatum Weak
TS28: fimbria hippocampus Weak
TS28: hippocampus Weak
TS28: main olfactory bulb Weak
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
MGI 6.13 		The Jackson Laboratory