Gene Expression Data
Assay Details
Assay
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Reference:
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J:63172
Erskine L, et al., Retinal ganglion cell axon guidance in the mouse optic chiasm: expression and function of robos and slits. J Neurosci. 2000 Jul 1;20(13):4975-82
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7565485
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Gene symbol:
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Robo2
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Gene name:
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roundabout guidance receptor 2
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Probe:
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Robo2 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
2C:
embryonic day 12.5
(more )
Note: vibratome section
Specimen
2C:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS20: retina |
retinal ganglion cell |
Present |
Regionally restricted |
2C
|
Expressed in the dorsocentral region, where the first retinal ganglion cells to be born.
|
Specimen
2H/Q:
embryonic day 14.5
(more )
Note: vibratome section
Specimen
2H/Q:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: retina |
retinal ganglion cell |
Present |
Regionally restricted |
2Q
,
2H
|
Expressed throughout retinal ganglion cell layer. Expressed by most cells in the layer.
|
Specimen
2M:
embryonic day 17.5
(more )
Note: vibratome section
Specimen
2M:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS25: retina layer |
retinal ganglion cell |
Present |
Regionally restricted |
2M
|
Expression was maintained within the retinal ganglion cell layer.
|
Specimen
3I:
embryonic day 12.5
(more )
Note: vibratome section
Specimen
3I:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: diencephalon |
Present |
Regionally restricted |
3I
|
Expressed in the CD44/SSEA (Fut4) neurons, which were posterior to the optic chiasm.
|
Specimen
3J:
embryonic day 12.5
(more )
Note: vibratome section
Specimen
3J:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: diencephalon |
Present |
Regionally restricted |
3J
|
Expressed in the CD44/SSEA (Fut4) neurons, which were posterior to the optic chiasm.
|
Specimen
3K:
embryonic day 12.5
(more )
Note: vibratome section
Specimen
3K:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: diencephalon |
Present |
Regionally restricted |
3K
|
Expressed in the CD44/SSEA (Fut4) neurons, which were posterior to the optic chiasm.
|
Specimen
3L:
embryonic day 12.5
(more )
Note: vibratome section
Specimen
3L:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: diencephalon |
Present |
Regionally restricted |
3L
|
Expressed in the CD44/SSEA (Fut4) neurons, which were posterior to the optic chiasm.
|
Specimen
4I:
embryonic day 14.5
(more )
Note: vibratome section
Specimen
4I:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: diencephalon |
Present |
Regionally restricted |
4I
|
Expressed in the CD44/SSEA (Fut4) neurons.
|
Specimen
4J:
embryonic day 14.5
(more )
Note: vibratome section
Specimen
4J:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: diencephalon |
Present |
Regionally restricted |
4J
|
Expressed in the CD44/SSEA (Fut4) neurons.
|
Specimen
4K:
embryonic day 14.5
(more )
Note: vibratome section
Specimen
4K:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: diencephalon |
Present |
Regionally restricted |
4K
|
Expressed in the CD44/SSEA (Fut4) neurons.
|
Specimen
4L:
embryonic day 14.5
(more )
Note: vibratome section
Specimen
4L:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: diencephalon |
Present |
Regionally restricted |
4L
|
Expressed in the CD44/SSEA (Fut4) neurons.
|
Specimen
5C:
embryonic day 17.5
(more )
Note: vibratome section
Specimen
5C:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: diencephalon |
Present |
Regionally restricted |
5C
|
Expressed in the CD44/SSEA (Fut4) neurons.
|
Specimen
not shown:
embryonic day 15.5
(more )
Note: vibratome section
Specimen
not shown:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
vibratome section
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS23: retina layer |
retinal ganglion cell |
Present |
Regionally restricted |
|
Expressed throughout retinal ganglion cell layer.
|
TS23: diencephalon |
|
Present |
Regionally restricted |
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Expressed in the CD44/SSEA (Fut4) neurons.
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