Gene Expression Data
Assay Details
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Assay
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Reference:
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J:63172
Erskine L, et al., Retinal ganglion cell axon guidance in the mouse optic chiasm: expression and function of robos and slits. J Neurosci. 2000 Jul 1;20(13):4975-82
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7565486
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Gene symbol:
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Slit1
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Gene name:
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slit guidance ligand 1
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Probe:
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Slit1 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
2D:
embryonic day 12.5
(more  )
Note: vibratome section
Specimen
2D:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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vibratome section
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: retina |
retinal ganglion cell |
Present |
Regionally restricted |
2D
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Expressed in the dorsocentral region, where the first retinal ganglion cells to be born.
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Specimen
2I:
embryonic day 14.5
(more )
Note: vibratome section
Specimen
2I:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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vibratome section
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: retina |
retinal ganglion cell |
Present |
Regionally restricted |
2I
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Expressed throughout retinal ganglion cell layer. Expressed by most cells in the layer in a high ventral, low dorsal gradient.
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Specimen
2N:
embryonic day 17.5
(more )
Note: vibratome section
Specimen
2N:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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vibratome section
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS25: retina layer |
retinal ganglion cell |
Weak |
Regionally restricted |
2N
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Very weakly expressed at the retinal ganglion cell layer.
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Specimen
3M:
embryonic day 12.5
(more )
Note: vibratome section
Specimen
3M:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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vibratome section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon |
Present |
Regionally restricted |
3M
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Expressed around the junction of the optic nerve and the brain, with strongest expression dorsal to the site at which the optic stalk joins the diencephalon.
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Specimen
3N:
embryonic day 12.5
(more )
Note: vibratome section
Specimen
3N:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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vibratome section
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon |
Present |
Regionally restricted |
3N
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Expressed around the junction of the optic nerve and the brain, with strongest expression dorsal to the site at which the optic stalk joins the diencephalon.
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Specimen
3O:
embryonic day 12.5
(more )
Note: vibratome section
Specimen
3O:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
|
vibratome section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon |
Present |
Regionally restricted |
3O
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Expressed around the junction of the optic nerve and the brain, with strongest expression dorsal to the site at which the optic stalk joins the diencephalon. In the more dorsal region, expression was some distance posterior to the axons. Also weakly expressed in a subset of the CD44/SSEA(Fut4) neurons.
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Specimen
3P:
embryonic day 12.5
(more )
Note: vibratome section
Specimen
3P:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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|
Note:
|
vibratome section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: diencephalon |
Present |
Regionally restricted |
3P
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Expressed around the junction of the optic nerve and the brain, with strongest expression dorsal to the site at which the optic stalk joins the diencephalon. More ventrally, expressed in a region directly adjacent to the path taken by the axons. Also weakly expressed in a subset of the CD44/SSEA(Fut4) neurons.
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Specimen
4M:
embryonic day 14.5
(more )
Note: vibratome section
Specimen
4M:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
|
vibratome section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: diencephalon |
Present |
Regionally restricted |
4M
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Expressed around the junction of the optic nerves and the brain, and in the CD44/SSEA (Fut4) neurons.
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Specimen
4N:
embryonic day 14.5
(more )
Note: vibratome section
Specimen
4N:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
|
Note:
|
vibratome section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: diencephalon |
Present |
Regionally restricted |
4N
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Expressed around the junction of the optic nerves and the brain, and in the CD44/SSEA (Fut4) neurons.
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Specimen
4O:
embryonic day 14.5
(more )
Note: vibratome section
Specimen
4O:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
vibratome section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: diencephalon |
Present |
Regionally restricted |
4O
|
Expressed around the junction of the optic nerves and the brain, and in the CD44/SSEA (Fut4) neurons.
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Specimen
4P:
embryonic day 14.5
(more )
Note: vibratome section
Specimen
4P:
(close )
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Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
vibratome section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: diencephalon |
Present |
Regionally restricted |
4P
|
Expressed around the junction of the optic nerves and the brain, and in the CD44/SSEA (Fut4) neurons.
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Specimen
5D:
embryonic day 17.5
(more )
Note: vibratome section
Specimen
5D:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
vibratome section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: diencephalon |
Weak |
Regionally restricted |
5D
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Only weakly expressed in the CD44/SSEA (Fut4) neurons.
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Specimen
not shown:
embryonic day 15.5
(more )
Note: vibratome section
Specimen
not shown:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
vibratome section
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: retina layer |
retinal ganglion cell |
Present |
Regionally restricted |
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Expressed throughout retinal ganglion cell layer in a high ventral, low dorsal gradient.
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| TS23: diencephalon |
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Present |
Regionally restricted |
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Expressed around the junction of the optic nerves and the brain, and in the CD44/SSEA (Fut4) neurons.
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