Probe:	Slit2 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS20: retina	Absent		2E

Structure	Cell Type	Level	Pattern	Image	Note
TS22: retina	retinal ganglion cell	Present	Regionally restricted	2J	Expressed throughout retinal ganglion cell layer. Expressed by most cells in the layer.

Structure	Level	Pattern	Image	Note
TS25: retina layer	Present	Regionally restricted	2R , 2O	Expressed at the inner nuclear layer.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Strong	Regionally restricted	3Q	Expressed at the ventral midline in the region in which the axons enter the brain and turn to grow ventrally, including the position of the glial knot, a glial structure at the midline of the diencephalon that has been proposed to delimit the pathway of the ingrowing axons.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Strong	Regionally restricted	3R	Expressed at the ventral midline in the region in which the axons enter the brain and turn to grow ventrally.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Strong	Regionally restricted	3S	Expressed at the ventral midline in the region in which the axons enter the brain and turn to grow ventrally (see 3Q and 3R).

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Strong	Regionally restricted	3T	Expressed at the ventral midline in the region in which the axons enter the brain and turn to grow ventrally (see 3Q and 3R).

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	4Q	Expression was maintained at the ventral midline, in a region directly dorsal to the site of axon divergence.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	4R	Expression was maintained at the ventral midline, in a region directly dorsal to the site of axon divergence.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	4S	Expression was maintained at the ventral midline, in a region directly dorsal to the site of axon divergence. No expression at the more ventral level at which the axons cross the midline.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	4T	Expression was maintained at the ventral midline, in a region directly dorsal to the site of axon divergence. No expression at the more ventral level at which the axons cross the midline.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	4W	Expression was maintained at the ventral midline, in a region directly dorsal to the site of axon divergence. Expressed by a subset of the midline radial glial cells (compared with 4V).

Structure	Level	Pattern	Image	Note
TS25: diencephalon	Present	Regionally restricted	5E	Expression was maintained at the ventral midline, in a region directly dorsal to the site of axon divergence. No expression at the more ventral level at which the axons cross the midline.

Structure	Cell Type	Level	Pattern	Image	Note
TS23: retina layer	retinal ganglion cell	Present	Regionally restricted		Expressed throughout retinal ganglion cell layer.
TS23: diencephalon		Present	Regionally restricted		Expression was maintained at the ventral midline, in a region directly dorsal to the site of axon divergence. No expression at the more ventral level at which the axons cross the midline.