Gene Expression Data
Assay Details
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Assay
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Reference:
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J:67383
Britsch S, et al., The transcription factor Sox10 is a key regulator of peripheral glial development. Genes Dev. 2001 Jan 1;15(1):66-78
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7569244
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Gene symbol:
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Kit
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Gene name:
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KIT proto-oncogene receptor tyrosine kinase
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Probe:
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pGEM-ckit
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Probe preparation:
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Antisense RNA
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Results
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Specimen
7a:
embryonic day 12.5
(more  )
Note: Quantified in Fig 7g.
Specimen
7a:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C3HeB/FeJ * C57BL/6J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Quantified in Fig 7g.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: migrating neural crest |
melanocyte |
Present |
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7a
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Expressed in a stream of positive melanocytes along the dorso-lateral pathway.
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Specimen
7c:
embryonic day 12.5; Sox10Dom/ Sox10Dom
(more )
Note: Quantified in Fig 7g.
Specimen
7c:
(close )
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Genetic Background:
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involves: C3HeB/FeJLe * C57BL/6JLe
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Mutant Allele(s):
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Sox10Dom/Sox10Dom
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Quantified in Fig 7g.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: migrating neural crest |
melanocyte |
Present |
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7c
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The numbers of positive cells were reduced by half, compared with the wild-type.
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Specimen
7e:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C3HeB/FeJ * C57BL/6J
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Mutant Allele(s):
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Sox10tm1Weg/Sox10tm1Weg
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Quantified in Fig 7g.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: migrating neural crest |
melanocyte |
Present |
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7e
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Pronounced reduction, contained only 24% of the cells compared with wild-type embryos.
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Analysis Tools