Wnt10b RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:231699 Voutilainen M, et al., Ectodysplasin/NF-kappaB Promotes Mammary Cell Fate via Wnt/beta-catenin Pathway. PLoS Genet. 2015 Nov;11(11):e1005676
Assay type:	RNA in situ
MGI Accession ID:	MGI:7574493
Gene symbol:	Wnt10b
Gene name:	wingless-type MMTV integration site family, member 10B

Probe:	Wnt10b cDNA5
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

1G left ©

Specimen 1G control: embryonic day 11.25 (more

)

Structure	Level	Pattern	Image	Note
TS19: mammary placode	Strong		1G left	Expressed in the emerging mammary placodes, as well as anterior to the fore limb bud.

4C left ©

Specimen 4C control: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: mammary bud	Present		4C left

4C right ©

Specimen 4C IkappaBalphadeltaN: embryonic day 12.5; Ctnnb1^{tm1(Nfkbia)Rsu}/Ctnnb1^{tm1(Nfkbia)Rsu} (more

)

Structure	Level	Pattern	Image	Note
TS20: mammary bud	Present		4C right	Somewhat smaller mammary buds compared with the control.

5B wt ©

Specimen 5B wt: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: mammary bud	Present		5B wt

Specimen 5B IkappaBalphadeltaN: embryonic day 13.5; Ctnnb1^{tm1(Nfkbia)Rsu}/Ctnnb1^{tm1(Nfkbia)Rsu} (more

)

Structure	Level	Pattern	Image	Note
TS21: mammary bud	Present		5B IkbadeltaN	Slightly downregulated expression compared with the wild-type.

7A Eda-/- ©

Specimen 7A Eda-/-: embryonic day 12.5; Eda^Ta/Eda^Ta (more

)

Structure	Level	Pattern	Image	Note
TS20: mammary bud	Present		7A Eda-/-	Reduced expression compared with the wild-type.

7A wt ©

Specimen 7A wt: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: mammary bud	Present		7A wt

S3_wt ©

Specimen S3 wt: embryonic day 13.75 (more

)

Structure	Level	Pattern	Image	Note
TS22: mammary bud	Present		S3_wt

S3_Foxi3-/- ©

Specimen S3 Foxi3-/-: embryonic day 13.75; Foxi3^tm1.2Akg/Foxi3^tm1.2Akg (more

)

Structure	Level	Pattern	Image	Note
TS22: mammary bud	Present		S3_Foxi3-/-	Similar as in the wild-type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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