Gene Expression Data
Assay Details
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Assay
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Reference:
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J:309045
Garcia-Guillen IM, et al., Netrin-1/DCC Signaling Differentially Regulates the Migration of Pax7, Nkx6.1, Irx2, Otp, and Otx2 Cell Populations in the Developing Interpeduncular Nucleus. Front Cell Dev Biol. 2020;8:588851
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7581068
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Gene symbol:
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Pax7
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Gene name:
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paired box 7
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Probe:
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RIKEN clone 5330440B08
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A:
embryonic day 15.5
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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Swiss albino
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: hindbrain mantle layer |
Present |
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1A
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Positive cells were migrating in the mantle layer, and began to reach the median interpeduncular nucleus.
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Specimen
1B:
embryonic day 18.5
(more )
Specimen
1B:
(close )
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Genetic Background:
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Swiss albino
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: interpeduncular nucleus |
Strong |
Regionally restricted |
1B
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Expressed mainly in the interpeduncular rostral and caudal region, but not in the prodromal region.
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| TS26: hindbrain |
Strong |
Regionally restricted |
1B
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Expressed in a large population of cells migrating en route to that nucleus.
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Specimen
3E:
embryonic day 18.5
(more )
Note: Medial region.
Specimen
3E:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Medial region.
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Specimen
3F:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Dcctm1Wbg/Dcctm1Wbg
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Medial region.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: interpeduncular nucleus |
Trace |
Regionally restricted |
3F
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The rostral expression was almost completely disappeared from the interpeduncular rostral, with only a few cells arriving at their destination.
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| TS26: hindbrain |
Present |
Patchy |
3F
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Expressed in patches of cells that appeared to have halted their migration to the interpeduncular nucleus.
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Specimen
3G:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Dcctm1Wbg/Dcctm1Wbg
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Medio-lateral region.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: interpeduncular nucleus |
Weak |
Regionally restricted |
3G
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The rostral expression was almost completely disappeared from the interpeduncular rostral, with only a few cells arriving at their destination. The caudal population seemed to be less severely affected, since more positive cells reached their correct location in the interpeduncular caudal.
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| TS26: hindbrain |
Present |
Patchy |
3G
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Expressed in patches of cells that appeared to have halted their migration to the interpeduncular nucleus.
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Specimen
not shown:
embryonic day 18.5
(more )
Note: Medio-lateral region.
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Note:
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Medio-lateral region.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: interpeduncular nucleus |
Present |
Regionally restricted |
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Expressed in the entire interpeduncular rostral region, and the medio-lateral part of the interpeduncular caudal region.
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Analysis Tools