Antibody:

anti-Myo6

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlear duct	cochlea auditory hair cell	Present	Regionally restricted	2C Prdm16cGT/+ Base	Expression was detected in hair cells in the base of the cochlear duct.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlear duct	cochlea auditory hair cell	Present	Regionally restricted	2C Prdm16cGT/cGT Base	Expression was detected in hair cells in the base of the cochlear duct.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlear duct	cochlea auditory hair cell	Present	Regionally restricted	2C Prdm16cGT/+ Middle	Expression was detected in hair cells in the middle of the cochlear duct.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlear duct	cochlea auditory hair cell	Present	Regionally restricted	2C Prdm16cGT/cGT Middle	Expression was detected in hair cells in the middle of the cochlear duct.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlear duct	cochlea auditory hair cell	Present	Regionally restricted	2C Prdm16cGT/+ Apex	Expression was detected in hair cells in the apex of the cochlear duct.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlear duct	cochlea auditory hair cell	Present	Regionally restricted	2C Prdm16cGT/cGT Apex	Expression was detected in hair cells in the apex of the cochlear duct. Staining showed a higher density of hair cells in the apical turn of Prdm16<cGT> null cochlea.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlear duct epithelium	cochlear inner hair cell	Present		3A Prdm16cGT/+
TS27: cochlear duct epithelium	cochlear outer hair cell	Present		3A Prdm16cGT/+

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlear duct epithelium	cochlear inner hair cell	Present		3A Prdm16cGT/cGT
TS27: cochlear duct epithelium	cochlear outer hair cell	Present		3A Prdm16cGT/cGT

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlear duct epithelium	cochlear inner hair cell	Present	Regionally restricted	3A Prdm16cGT/+ Base	Expression was detected in hair cells in the base of the cochlear duct.
TS27: cochlear duct epithelium	cochlear outer hair cell	Present	Regionally restricted	3A Prdm16cGT/+ Base	Expression was detected in hair cells in the base of the cochlear duct.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlear duct epithelium	cochlear inner hair cell	Present	Regionally restricted	3A Prdm16cGT/cGT Base	Expression was detected in supporting cells in the base of the cochlear duct with no change in density relative to control.
TS27: cochlear duct epithelium	cochlear outer hair cell	Present	Regionally restricted	3A Prdm16cGT/cGT Base	Expression was detected in supporting cells in the base of the cochlear duct with no change in density relative to control.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlear duct epithelium	cochlear inner hair cell	Present	Regionally restricted	3A Prdm16cGT/+ Middle	Expression was detected in hair cells in the middle of the cochlear duct.
TS27: cochlear duct epithelium	cochlear outer hair cell	Present	Regionally restricted	3A Prdm16cGT/+ Middle	Expression was detected in hair cells in the middle of the cochlear duct.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlear duct epithelium	cochlear inner hair cell	Present	Regionally restricted	3A Prdm16cGT/cGT Middle	Expression was detected in supporting cells in the middle of the cochlear duct with no change in density relative to control.
TS27: cochlear duct epithelium	cochlear outer hair cell	Present	Regionally restricted	3A Prdm16cGT/cGT Middle	Expression was detected in supporting cells in the middle of the cochlear duct with no change in density relative to control.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlear duct epithelium	cochlear inner hair cell	Present	Regionally restricted	3A Prdm16cGT/+ Apex	Expression was detected in hair cells in the apex of the cochlear duct.
TS27: cochlear duct epithelium	cochlear outer hair cell	Present	Regionally restricted	3A Prdm16cGT/+ Apex	Expression was detected in hair cells in the apex of the cochlear duct.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlear duct epithelium	cochlear inner hair cell	Present	Regionally restricted	3A Prdm16cGT/cGT Apex	Expression was detected in inner hair cells in the apex of the cochlear duct with a density around 150% relative to control.
TS27: cochlear duct epithelium	cochlear outer hair cell	Present	Regionally restricted	3A Prdm16cGT/cGT Apex	Expression was detected in outer hair cells in the apex of the cochlear duct with a density around 140% relative to control.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlear duct epithelium	cochlear inner hair cell	Present		4A Prdm16cGT/+ Base Myo6 , 4A Prdm16cGT/+ Base Merge , 4A Prdm16cGT/+
TS27: cochlear duct epithelium	cochlear outer hair cell	Present		4A Prdm16cGT/+ Base Myo6 , 4A Prdm16cGT/+ Base Merge , 4A Prdm16cGT/+

Structure	Cell Type	Level	Pattern	Image	Note
TS27: cochlear duct epithelium	cochlear inner hair cell	Present		4A Prdm16cGT/cGT , 4A Prdm16cGT/cGT Base Merge , 4A Prdm16cGT/cGT Base Myo6
TS27: cochlear duct epithelium	cochlear outer hair cell	Present		4A Prdm16cGT/cGT , 4A Prdm16cGT/cGT Base Merge , 4A Prdm16cGT/cGT Base Myo6
TS27: greater epithelial ridge	cochlea auditory hair cell	Present	Single cells	4A Prdm16cGT/cGT Base Merge , 4A Prdm16cGT/cGT Base Myo6 , 4A Prdm16cGT/cGT Middle Merge , 4A Prdm16cGT/cGT Middle Myo6	Ectopic hair cells were detected in the Kolliker's organ. The number of ectopic HCs in the whole cochlea ranged from 6 to 32 cells/cochlea, and they occurred either in clusters of 4 to 5 cells or as individual cells. Tubb3 immunostaining revealed that the ectopic cells were innervated with nerve fibers running through the Kolliker's organ.