Gene Expression Data
Assay Details
Assay
|
Reference:
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J:316184
Hussein MT, et al., Spatiotemporal expression of sonic hedgehog signalling molecules in the embryonic mesencephalic dopaminergic neurons. Gene Expr Patterns. 2021 Dec;42:119217
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7596080
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Gene symbol:
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Ptch1
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Gene name:
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patched 1
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Probe:
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Ptch1 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A:
embryonic day 12.0
(more )
Specimen
1A:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: midbrain |
Present |
Regionally restricted |
1A
|
In this rostral section there are three main faint bands of expression along the
lateral parts, with a more prominent central one.
|
Specimen
1B:
embryonic day 12.0
(more )
Specimen
1B:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: midbrain |
Present |
Regionally restricted |
1B
|
Expression is in the middle lateral domain.
|
Specimen
1C:
embryonic day 12.0
(more )
Specimen
1C:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: midbrain |
Present |
Regionally restricted |
1C
|
In this more caudal section expression is in the middle lateral domain and is expanding dorsally.
|
Specimen
2B:
embryonic day 12.0
(more )
Specimen
2B:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
2E:
embryonic day 12.0
(more )
Specimen
2E:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
3A:
embryonic day 14.0
(more )
Specimen
3A:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: midbrain |
Present |
Regionally restricted |
3A
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Expression is in the ventrolateral domain.
|
Specimen
4A:
embryonic day 14.0
(more )
Specimen
4A:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: midbrain |
Present |
Regionally restricted |
4A
|
Expression is ventral.
|
Specimen
4B:
embryonic day 14.0
(more )
Specimen
4B:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: midbrain |
Present |
Regionally restricted |
4B
|
Expression is ventral.
|
Specimen
6B:
embryonic day 18.0
(more )
Specimen
6B:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 18.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
|