Gene Expression Data
Assay Details
Assay
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Reference:
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J:316184
Hussein MT, et al., Spatiotemporal expression of sonic hedgehog signalling molecules in the embryonic mesencephalic dopaminergic neurons. Gene Expr Patterns. 2021 Dec;42:119217
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7596083
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Gene symbol:
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Gli3
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Gene name:
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GLI-Kruppel family member GLI3
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Probe:
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Gli3 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1J:
embryonic day 12.0
(more )
Specimen
1J:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
embryonic day 12.0
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Sex:
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Not Specified
|
Type:
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section
|
Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: midbrain |
Present |
Regionally restricted |
1J
|
In this rostral section expression is diffuse in the dorsal half.
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Specimen
1K:
embryonic day 12.0
(more )
Specimen
1K:
(close )
Genetic Background:
|
C57BL/6J
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Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: midbrain |
Present |
Regionally restricted |
1K
|
In this middle section expression is diffuse in the dorsal half.
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Specimen
1L:
embryonic day 12.0
(more )
Specimen
1L:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: midbrain |
Present |
Regionally restricted |
1L
|
In this more caudal section expression is in the middle lateral domain and is expanding dorsally.
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Specimen
3D:
embryonic day 14.0
(more )
Specimen
3D:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: midbrain |
Present |
Regionally restricted |
3D
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Expression is confined to the dorsolateral wall of the aqueduct.
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TS22: pons |
Present |
Regionally restricted |
3D
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Expression is in the red nucleus.
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Specimen
3K:
embryonic day 14.0
(more )
Specimen
3K:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
4C:
embryonic day 14.0
(more )
Specimen
4C:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: midbrain |
Present |
Regionally restricted |
4C
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Expression is dorsolateral.
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Specimen
4D:
embryonic day 14.0
(more )
Specimen
4D:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 14.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: midbrain |
Present |
Regionally restricted |
4D
|
Expression is dorsolateral.
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Specimen
6A:
embryonic day 18.0
(more )
Specimen
6A:
(close )
Genetic Background:
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C57BL/6J
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Age:
|
embryonic day 18.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
|