Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:262420
Szenker-Ravi E, et al., RSPO2 inhibition of RNF43 and ZNRF3 governs limb development independently of LGR4/5/6. Nature. 2018 May;557(7706):564-569
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:7596255
|
Gene symbol:
|
Lgr6
|
Gene name:
|
leucine-rich repeat-containing G protein-coupled receptor 6
|
Probe:
|
Mm-Lgr6 (404961)
|
Probe preparation:
|
Antisense DNA
|
Visualized with:
|
Chromogenic
|
|
Results
|
Specimen
2h Lgr6/Rspo2 (upper):
embryonic day 14.5
(more )
Note: Double labeled: blue - Lgr6; pink - Rspo2 (assay MGI:7596266).
Specimen
2h Lgr6/Rspo2 (upper):
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: blue - Lgr6; pink - Rspo2 (assay MGI:7596266).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: forelimb epidermis |
Present |
Regionally restricted |
2h Lgr6/Rspo2 (upper)
|
Expression co-localized with Rspo2 in apical ectodermal ridge.
|
Specimen
2h Lgr6/Rspo2 l(ower):
embryonic day 14.5
(more )
Note: Double labeled: blue - Lgr6; pink - Rspo2 (assay MGI:7596266).
Specimen
2h Lgr6/Rspo2 l(ower):
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: blue - Lgr6; pink - Rspo2 (assay MGI:7596266).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: forelimb epidermis |
Present |
Regionally restricted |
2h Lgr6/Rspo2 (lower)
|
Expression co-localized with Rspo2 in apical ectodermal ridge.
|
Specimen
ED5f Lgr6/Rspo2 (upper):
embryonic day 14.5
(more )
Note: Double labeled: blue - Lgr6; pink - Rspo2 (assay MGI:7596266).
Specimen
ED5f Lgr6/Rspo2 (upper):
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: blue - Lgr6; pink - Rspo2 (assay MGI:7596266).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: lung |
smooth muscle cell |
Strong |
Regionally restricted |
ED5f Lgr6/Rspo2 (upper)
|
Robust expression in smooth muscle cells.
|
Specimen
ED5f Lgr6/Rspo2 (lower):
embryonic day 14.5
(more )
Note: Double labeled: blue - Lgr6; pink - Rspo2 (assay MGI:7596266).
Specimen
ED5f Lgr6/Rspo2 (lower):
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: blue - Lgr6; pink - Rspo2 (assay MGI:7596266).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: lung |
smooth muscle cell |
Strong |
Regionally restricted |
ED5f Lgr6/Rspo2 (lower)
|
Robust expression in smooth muscle cells.
|
|