Gene Expression Data
Assay Details
Assay
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Reference:
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J:270646
Limi S, et al., Transcriptional burst fraction and size dynamics during lens fiber cell differentiation and detailed insights into the denucleation process. J Biol Chem. 2018 Aug 24;293(34):13176-13190
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7618443
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Gene symbol:
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Polr2a
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Gene name:
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polymerase (RNA) II (DNA directed) polypeptide A
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Results
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Specimen
9 region a WT:
postnatal day 1
(more )
Note: This region represents the least differentiated lens tissue.
Specimen
9 region a WT:
(close )
Genetic Background:
|
FVB
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
This region represents the least differentiated lens tissue.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
9A a WT
|
There is punctate staining in lens fiber cells that include regions a, b, and c.
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Specimen
9 region b WT:
postnatal day 1
(more )
Note: This region represents the intermediate differentiated lens tissue.
Specimen
9 region b WT:
(close )
Genetic Background:
|
FVB
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
This region represents the intermediate differentiated lens tissue.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
9A b WT
|
There is punctate staining in lens fiber cells that include regions a, b, and c.
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Specimen
9 region c WT:
postnatal day 1
(more )
Note: This region represents the most highly differentiated lens tissue.
Specimen
9 region c WT:
(close )
Genetic Background:
|
FVB
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
This region represents the most highly differentiated lens tissue.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
9A c WT
|
There is punctate staining in lens fiber cells that include regions a, b, and c.
|
Specimen
9 region a Snf2hnull:
postnatal day 1; Smarca5tm2.1Ais/ Smarca5tm2.1Ais
(more )
Note: This region represents the least differentiated lens tissue.
Specimen
9 region a Snf2hnull:
(close )
Genetic Background:
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involves: 129 * C57BL/6J * SJL
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Mutant Allele(s):
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Smarca5tm2.1Ais/Smarca5tm2.1Ais
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Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
This region represents the least differentiated lens tissue.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
9A a Snf2hnull
|
Expression is altered due to disorganized lens fibers in this mutant.
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Specimen
9 region b Snf2hnull:
postnatal day 1; Smarca5tm2.1Ais/ Smarca5tm2.1Ais
(more )
Note: This region represents the intermediate differentiated lens tissue.
Specimen
9 region b Snf2hnull:
(close )
Genetic Background:
|
involves: 129 * C57BL/6J * SJL
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Mutant Allele(s):
|
Smarca5tm2.1Ais/Smarca5tm2.1Ais
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
This region represents the intermediate differentiated lens tissue.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
9A b Snf2hnull
|
Expression is altered due to disorganized lens fibers in this mutant.
|
Specimen
9 region c Snf2hnull:
postnatal day 1; Smarca5tm2.1Ais/ Smarca5tm2.1Ais
(more )
Note: This region represents the most highly differentiated lens tissue.
Specimen
9 region c Snf2hnull:
(close )
Genetic Background:
|
involves: 129 * C57BL/6J * SJL
|
Mutant Allele(s):
|
Smarca5tm2.1Ais/Smarca5tm2.1Ais
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
This region represents the most highly differentiated lens tissue.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
9A c Snf2hnull
|
Expression is altered due to disorganized lens fibers in this mutant.
|
Specimen
S3a:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - beta-actin.
Specimen
S3a:
(close )
Genetic Background:
|
FVB
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Polr2a; red - beta-actin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S3a Pol II
,
S3a Merge
|
Expression is in transcription start sites in the nuclei.
|
Specimen
S3b:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - beta-actin.
Specimen
S3b:
(close )
Genetic Background:
|
FVB
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Polr2a; red - beta-actin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S3b Merge
,
S3b Pol II
|
Expression is in transcription start sites in the nuclei.
|
Specimen
S3c:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - beta-actin.
Specimen
S3c:
(close )
Genetic Background:
|
FVB
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Polr2a; red - beta-actin.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S3c Pol II
,
S3c Merge
|
Expression is in transcription start sites in the nuclei.
|
Specimen
S4a:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - Cryaa (assay MGI:7618431).
Specimen
S4a:
(close )
Genetic Background:
|
FVB
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Polr2a; red - Cryaa (assay MGI:7618431).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S4a Pol II
,
S4a Merge
|
Expression is in transcription start sites in the nuclei.
|
Specimen
S4b:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - Cryaa (assay MGI:7618431).
Specimen
S4b:
(close )
Genetic Background:
|
FVB
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Polr2a; red - Cryaa (assay MGI:7618431).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S4b Merge
,
S4b Pol II
|
Expression is in transcription start sites in the nuclei.
|
Specimen
S4c:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - Cryaa (assay MGI:7618431).
Specimen
S4c:
(close )
Genetic Background:
|
FVB
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Polr2a; red - Cryaa (assay MGI:7618431).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S4c Merge
,
S4c Pol II
|
Expression is in transcription start sites in the nuclei.
|
Specimen
S5a:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - Cryba1 (assay MGI:7618449).
Specimen
S5a:
(close )
Genetic Background:
|
FVB
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Polr2a; red - Cryba1 (assay MGI:7618449).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S5a Merge
,
S5a Pol II
|
Expression is in transcription start sites in the nuclei.
|
Specimen
S5b:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - Cryba1 (assay MGI:7618449).
Specimen
S5b:
(close )
Genetic Background:
|
FVB
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Polr2a; red - Cryba1 (assay MGI:7618449).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S5b Pol II
,
S5b Merge
|
Expression is in transcription start sites in the nuclei.
|
Specimen
S5c:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - Cryba1 (assay MGI:7618449).
Specimen
S5c:
(close )
Genetic Background:
|
FVB
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Polr2a; red - Cryba1 (assay MGI:7618449).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S5c Merge
,
S5c Pol II
|
Expression is in transcription start sites in the nuclei.
|
Specimen
S6a:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - Cryga (assay MGI:7618450).
Specimen
S6a:
(close )
Genetic Background:
|
FVB
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Polr2a; red - Cryga (assay MGI:7618450).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S6a Pol II
,
S6a Merge
|
Expression is in transcription start sites in the nuclei.
|
Specimen
S6b:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - Cryga (assay MGI:7618450).
Specimen
S6b:
(close )
Genetic Background:
|
FVB
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Polr2a; red - Cryga (assay MGI:7618450).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S6b Pol II
,
S6b Merge
|
Expression is in transcription start sites in the nuclei.
|
Specimen
S6c:
postnatal day 1
(more )
Note: Double labeled: green - Polr2a; red - Cryga (assay MGI:7618450).
Specimen
S6c:
(close )
Genetic Background:
|
FVB
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Polr2a; red - Cryga (assay MGI:7618450).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: lens fiber |
lens fiber cell |
Present |
Punctate |
S6c Merge
,
S6c Pol II
|
Expression is in transcription start sites in the nuclei.
|
|