Probe:	Hes1 probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Regionally restricted	2A Hes1	Authors report regionalized expression overlapping with distribution of transgene expession.
TS15: nasal process ectoderm	Present		2A Hes1	Expression in nasal ectoderm.
TS15: branchial arch	Present	Regionally restricted	2A Hes1	Expression in ventral pharyngeal region.
TS15: paraxial mesenchyme	Present	Regionally restricted	2A Hes1	Expression in segmental intersomitic region.
TS15: neural tube	Present	Regionally restricted	2A Hes1	Expression in ventral neural tube.
TS15: forelimb bud	Present		2A Hes1
TS15: future midbrain	Present		2A Hes1
TS15: tail	Present		2A Hes1

Structure	Level	Pattern	Image	Note
TS17: embryo	Present	Regionally restricted	3A Hes1 (left)	Authors report expression overlapping with distribution of transgene expression.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	3A Hes1 Level a	Expression in neural tube, including specific population of neurons in ventral neural tube.
TS17: diencephalon	Present		3A Hes1 Level a

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	3A Hes1 Level b	Expression in neural tube, including specific population of neurons in ventral neural tube.
TS17: pharynx epithelium	Present		3A Hes1 Level b	Expression in pharyngeal region, including ectoderm.

Structure	Level	Pattern	Image	Note
TS20: forelimb interdigital region	Present		3B Hes1 (left)

Structure	Level	Pattern	Image	Note
TS20: lung epithelium	Present		3B Hes1 (right)

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Weak	Regionally restricted	4A Hes1 (left)	Low level expression in distal outflow tract, superior outflow tract wall and mesenchymal cells in outflow tract.

Structure	Level	Pattern	Image	Note
TS17: pericardium	Weak	Regionally restricted	4A Hes1 (right)	Low level expression in pericardial region.

Structure	Level	Pattern	Image	Note
TS13: pericardium	Present	Regionally restricted	4B Hes1 (left)	Expression in dorsal pericardial wall.

Structure	Level	Pattern	Image	Note
TS13: embryo mesoderm	Present	Regionally restricted	4B Hes1 (right)	Expression in mesoderm lateral to pharynx.

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present		4Ca Hes1	Expression in neuroepithelium.

Structure	Level	Pattern	Image	Note
TS13: embryo endoderm	Present	Regionally restricted	4Cb Hes1	Expression in endoderm and mesoderm lateral and ventral to pharynx, including second heart field.
TS13: embryo mesoderm	Present	Regionally restricted	4Cb Hes1	Expression in endoderm and mesoderm lateral and ventral to pharynx, including second heart field.
TS13: secondary heart field	Present		4Cb Hes1

Structure	Level	Pattern	Image	Note
TS13: embryo endoderm	Present	Regionally restricted	4Cc Hes1	Expression in endoderm and mesoderm lateral and ventral to pharynx, including second heart field.
TS13: embryo mesoderm	Present	Regionally restricted	4Cc Hes1	Expression in endoderm and mesoderm lateral and ventral to pharynx, including second heart field.
TS13: secondary heart field	Present		4Cc Hes1

Structure	Level	Pattern	Image	Note
TS13: embryo endoderm	Present	Regionally restricted	4Cd Hes1	Expression in endoderm and mesoderm lateral and ventral to pharynx, including second heart field.
TS13: embryo mesoderm	Present	Regionally restricted	4Cd Hes1	Expression in endoderm and mesoderm lateral and ventral to pharynx, including second heart field.
TS13: secondary heart field	Present		4Cd Hes1

Structure	Level	Pattern	Image	Note
TS13: embryo endoderm	Present	Regionally restricted	4Ce Hes1	Expression in endoderm lateral and ventral to pharynx.